| GeneLibs

题目:: Cardiac side population cells and Sca-1-positive cells.
作者:: Nagai(Toshio),Matsuura(Katsuhisa),Komuro(Issei)
状态:: 发布时间2013-06-28 , 更新时间 2013-06-28
期刊:: Methods Mol Biol
摘要:: Since the resident cardiac stem/progenitor cells were discovered, their ability to maintain the architecture and functional integrity of adult heart has been broadly explored. The methods for isolation and purification of the cardiac stem cells are crucial for the precise analysis of their developmental origin and intrinsic potential as tissue stem cells. Stem cell antigen-1 (Sca-1) is one of the useful cell surface markers to purify the cardiac progenitor cells. Another purification strategy is based on the high efflux ability of the dye, which is a common feature of tissue stem cells. These dye-extruding cells have been called side population cells because they locate in the side of dye-retaining cells after fluorescent cell sorting. In this chapter, we describe the methodology for the isolation of cardiac SP cells and Sca-1 positive cells.
语言:: eng
DOI:: 10.1007/978-1-62703-511-8_5

文献库文献相关信息