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PMID: 238029 Published · ppublish English Journal Article

Acidification in the rat proximal jejunum.

The Journal of physiology ·Vol. 245 ·No. 2 ·1975-02-00 ·Pages 333-50

Blair JA, Lucas ML, Matty AJ

Abstract

1. Production of hydrogen ions by the rat proximal jejunum was investigated using the everted sac technique. 2. Acidification occurred in the absence of glucose, increasing on addition of glucose to reach a maximal value. An apparent Km of 1-78 mM was derived for the glucose-dependent process. 3. Acidification in the presence of glucose was inhibited by 10 mM-2:4-dinitrophenol, 10 mM phlorrhizin, 10 mM aminophylline and anaerobiosis. 4. Histamine, ethylenediamine tetraacetic acid (EDTA), ouabain and acetazolamide, compounds known to alter acid production in gastric mucosa had no effect on jejunal acidification. 5. Galactose and 3-O-methylglucose failed to increase acidification; in contrast, mannose and fructose did lead to increases, indicating metabolic origin of the hydrogen ions. 6. Serosal and mucosal lactate production were measured and the calculated percentage of hydrogen ions possibly derived from this source was shown to account for only a small proportion of acidification. 7. The greatest increase in acidification with minimal simultaneous production of lactate occurred with ATP which was shown not to enter intestinal tissues. 8. A hypothesis for acidification, that of the break-down at the mucosal surface of ATP from intracellular metabolic sources, is proposed and its relevance to the postulated microclimate is discussed.

MeSH Terms
Acetazolamide/pharmacology Adenosine Triphosphate/metabolism Aminophylline/pharmacology Anaerobiosis Animals Dinitrophenols/pharmacology Edetic Acid/pharmacology Fructose/pharmacology Galactose/pharmacology Glucose/pharmacology Histamine/pharmacology Hydrogen-Ion Concentration In Vitro Techniques Jejunum/drug effects,metabolism Lactates/metabolism Male Mannose/pharmacology Methylglucosides/pharmacology Ouabain/pharmacology Phlorhizin/pharmacology Rats
Chemicals
Dinitrophenols Lactates Methylglucosides Aminophylline Fructose Ouabain Histamine Adenosine Triphosphate Edetic Acid Phlorhizin Glucose Acetazolamide Mannose Galactose
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Blair J A
Lucas M L
Matty A J
References (67)
67 references, click to expand
  1. The sodium and potassium activated ATPase. II. Comparative study of intestinal epithelium and red cells.
    J Cell Physiol. 1966 Jun;67(3):487-500 PMID: 4225498
  2. Intestinal transport of fructose.
    Lancet. 1970 Oct 17;2(7677):827-8 PMID: 4196053
  3. The morphology of the polysaccharide coat of mammalian cells.
    Biochim Biophys Acta. 1972 Feb 14;265(1):85-113 PMID: 4111356
  4. Studies on transmural potentials in vitro in relation to intestinal absorption. IV. Phlorizin-sugar interactions in rat gut.
    Biochim Biophys Acta. 1967 Jul 3;135(3):496-506 PMID: 6048819
  5. Acidification of mucosal fluid by transport of bicarbonate ion in turtle bladders.
    Am J Physiol. 1966 May;210(5):997-1008 PMID: 5947277
  6. Transfer of propionate by rat small intestine in vitro.
    J Physiol. 1966 Jan;182(1):150-63 PMID: 5937409
  7. Cation control in human erythrocytes.
    J Physiol. 1949 May 15;108(3):247-63 PMID: 16991856
  8. [Absorption of sugars in relation to intestinal pH].
    Rev Esp Fisiol. 1950 Dec;6(4):255-69 PMID: 14844989
  9. Absorption of sugars from isolated surviving intestine.
    Arch Biochem Biophys. 1955 Jun;56(2):412-23 PMID: 14377592
  10. EFFECT OF IMIDAZOLES ON ACTIVE TRANSPORT BY GASTRIC MUCOSA AND URINARY BLADDER.
    Am J Physiol. 1965 Jun;208:1183-90 PMID: 14301376
  11. ELECTRICAL POTENTIALS ASSOCIATED WITH INTESTINAL SUGAR TRANSFER.
    J Physiol. 1964 Jun;171:316-38 PMID: 14191482
  12. CALCIUM EXCHANGE IN ISOLATED INTESTINAL VILLI.
    Biochim Biophys Acta. 1963 Sep 24;75:250-6 PMID: 14083906
  13. Sac of everted intestine technic for study of intestinal absorption in vitro.
    Methods Med Res. 1961;9:287-92 PMID: 14007674
  14. Effects of histamine, compound 48/80, atropine, and diamox, administered to gastric submucosa, on acid secretion in the rat.
    Gastroenterology. 1961 Dec;41:572-9 PMID: 13922896
  15. Effect of ouabain on acid secretion and electrolyte content of frog gastric mucosa.
    Proc Soc Exp Biol Med. 1962 Jul;110:613-5 PMID: 13883727
  16. Adenosine 3',5'-phosphate in biological materials. I. Purification and properties of cyclic 3',5'-nucleotide phosphodiesterase and use of this enzyme to characterize adenosine 3',5'-phosphate in human urine.
    J Biol Chem. 1962 Apr;237:1244-50 PMID: 13875173
  17. Observations on the changes in the potassium content of rat jejunal mucosa during absorption.
    Biochim Biophys Acta. 1962 May 7;59:249-51 PMID: 13873700
  18. Galactose I-phosphate in the intestinal tissue of the rat during galactose absorption.
    Biochim Biophys Acta. 1960 Dec 18;45:490-8 PMID: 13722741
  19. Studies on the mechanism of the intestinal absorption of sugars. III. Mutual inhibition, in vitro, between some actively transported sugars.
    Biochim Biophys Acta. 1960 Dec 18;45:477-82 PMID: 13696270
  20. Transfer of glucose and fluid by different parts of the small intestine of the rat.
    J Physiol. 1961 Jul;157:279-88 PMID: 13687343
  21. On the mechanism of intestinal absorption of drugs.
    J Pharmacol Exp Ther. 1959 Apr;125(4):275-82 PMID: 13642268
  22. The action of phlorrhizin on the intestinal transfer of glucose and water in vitro.
    J Physiol. 1958 Dec 30;144(3):387-402 PMID: 13621404
  23. Studies on the internal pH of large muscle and nerve fibres.
    J Physiol. 1958 Jun 18;142(1):22-62 PMID: 13564418
  24. Absorption of drugs from the rat small intestine.
    J Pharmacol Exp Ther. 1958 May;123(1):81-8 PMID: 13539795
  25. Acid-base changes across the wall of hamster and rat intestine.
    Biochim Biophys Acta. 1957 Apr;24(1):124-32 PMID: 13426210
  26. [Enzymic determination of L(+)-lactic acid].
    Biochem Z. 1957;328(7):509-21 PMID: 13426146
  27. On the role of hydrogen ion and potassium ion in the active transport of sodium across the isolated frog skin.
    J Cell Physiol. 1957 Feb;49(1):129-52 PMID: 13416346
  28. The fate of 3-O-14CH3-glucose in the rat.
    Biochim Biophys Acta. 1956 Oct;22(1):185-6 PMID: 13373862
  29. The hexokinase activity of the intestinal mucosa.
    Biochim Biophys Acta. 1956 Jan;19(1):144-52 PMID: 13304081
  30. Concentration gradients of lactate, hydrogen and some other ions across the intestine in vitro.
    Biochem J. 1954 Mar;56(3):521-7 PMID: 13140239
  31. The use of sacs of everted small intestine for the study of the transference of substances from the mucosal to the serosal surface.
    J Physiol. 1954 Jan;123(1):116-25 PMID: 13131249
  32. Lactate and hydrogen ion gradients developed across the rat intestine in vitro.
    Biochim Biophys Acta. 1953 Jul;11(3):448-9 PMID: 13093757
  33. Glucose movements across the wall of the rat small intestine.
    J Physiol. 1953 Feb 27;119(2-3):210-23 PMID: 13035745
  34. Characteristics of hydrogen ion transport in urinary bladder of water turtle.
    J Clin Invest. 1967 Oct;46(10):1531-40 PMID: 6061730
  35. Mechanism of acidification in turtle bladder.
    Fed Proc. 1967 Sep;26(5):1314-21 PMID: 6051312
  36. The polarographic determination of the respiration of the small intestine of the rat.
    Biochim Biophys Acta. 1965 Oct 18;107(3):397-404 PMID: 5867297
  37. Stimulation of the gastric mucosa by adenosine-3',5'-monophosphate.
    Fed Proc. 1965 Nov-Dec;24(6):1368-76 PMID: 5853155
  38. Papain membrane on a collodion matrix: preparation and enzymic behavior.
    Science. 1965 Nov 5;150(3697):758-60 PMID: 5844079
  39. Sodium pumps in the rat small intestine in relation to hexose transfer and metabolism.
    J Physiol. 1969 Oct;204(2):299-310 PMID: 5824640
  40. The simultaneous determination of acetoacetate and glucose in capillary blood.
    Clin Chim Acta. 1969 Jul;25(1):109-16 PMID: 5797112
  41. Papain--collodion membranes. II. Analysis of the kinetic behavior of enzymes immobilized in artificial membranes.
    Biochemistry. 1968 Dec;7(12):4518-32 PMID: 5700670
  42. Papain-collodion membranes. I. Preparation and properties.
    Biochemistry. 1968 Feb;7(2):486-500 PMID: 5642388
  43. [Studies of the modification of glucose resorption by various H+-ion concentrations].
    Z Gesamte Exp Med. 1967;144(1):14-23 PMID: 5592675
  44. Effect of buffer constituents and time on drug transfer across in vitro rat intestine.
    J Pharm Sci. 1971 Feb;60(2):234-8 PMID: 5572446
  45. Experimental diarrhea. 3. Bicarbonate transport in rat salmonella enterocolitis.
    Gastroenterology. 1971 Jun;60(6):1076-86 PMID: 5556912
  46. The production of a hydrogen ion gradient across the isolated frog skin. Quantitative aspects and the effect of acetazolamide.
    Biochim Biophys Acta. 1970 Jun 2;203(3):394-409 PMID: 5523739
  47. The use of gastric function tests by members of the British Society of Gastroenterology.
    Gut. 1970 Dec;11(12):1056 PMID: 5511784
  48. The effect of adenosine triphosphate on the transmural potential in rat small intestine.
    J Physiol. 1970 May;208(1):203-20 PMID: 5499760
  49. The transport of pteroylglutamic acid across the small intestine of the rat.
    Biochim Biophys Acta. 1970;219(1):37-46 PMID: 5473514
  50. [The influence of secretin on acid, electrolyte and pepsinogen secretion of the human stomach stimulated by methylxanthine (theophylline)].
    Klin Wochenschr. 1971 Nov 15;49(22):1231-4 PMID: 5132328
  51. Selective permeability of the small intestine for fructose.
    Am J Physiol. 1971 Oct;221(4):1051-6 PMID: 5111247
  52. CO 2 gradients and acidification by transport of HCO 3 in turtle bladders.
    Am J Physiol. 1972 Feb;222(2):272-81 PMID: 5058367
  53. Studies on epithelial cells isolated from guinea pig small intestine.
    J Cell Biol. 1971 Nov;51(21):452-64 PMID: 5000170
  54. Altered absorption of drugs from the rat small intestine by carbonic anhydrase inhibition.
    J Pharmacol Exp Ther. 1970 Aug;174(2):177-84 PMID: 4988984
  55. Effects of chelation of calcium on the gastric mucosal barrier.
    Gastroenterology. 1970 Aug;59(2):200-7 PMID: 4988581
  56. Carbonic anhydrase: chemistry, physiology, and inhibition.
    Physiol Rev. 1967 Oct;47(4):595-781 PMID: 4964060
  57. Absorption of folic acid by everted segments of rat jejunum.
    J Physiol. 1974 Feb;236(3):653-61 PMID: 4822579
  58. Changes in the adenine nucleotide content of preparations of the rat small intestine in vitro.
    J Physiol. 1973 Nov;235(1):183-96 PMID: 4778135
  59. Ouabain action on sodium, chloride and fluid transport in rabbit jejunum and ileum.
    Experientia. 1973 Oct 15;29(10):1243-4 PMID: 4758927
  60. Ionic basis of membrane potentials of epithelial cells in rat small intestine.
    J Physiol. 1972 Dec;227(1):217-31 PMID: 4646579
  61. Sodium transfer in bullfrog small intestine. Stimulation by exogenous ATP.
    Biochim Biophys Acta. 1972 Feb 11;255(2):663-74 PMID: 4536699
  62. Stimulation of intestinal mucosal adenyl cyclase by cholera enterotoxin and prostaglandins.
    J Clin Invest. 1971 Jun;50(6):1218-30 PMID: 4325309
  63. Na-K ATPase from lizard gastric mucosa: studies on Na-k ATPase XXXI.
    Pflugers Arch. 1972;334(2):141-53 PMID: 4262606
  64. Species differences in influence of divalent cations and of ouabain on ATPase activity on intestinal brush borders of rat and guinea pig.
    Biochem Pharmacol. 1971 Jun;20(6):1119-22 PMID: 4256124
  65. Evidence for the uptake of ATP by rat soleus muscle in vitro.
    Biochim Biophys Acta. 1970;196(2):320-6 PMID: 4244309
  66. Membrane adenosine triphosphatase and cation transport.
    Br Med Bull. 1968 May;24(2):165-9 PMID: 4231272
  67. The intestinal response to ischaemia.
    Naunyn Schmiedebergs Arch Pharmakol Exp Pathol. 1966;255(2):178-91 PMID: 4228176
Article Info
Journal
The Journal of physiology
Abbr.
J Physiol
ISSN
0022-3751
DOI
10.1113/jphysiol.1975.sp010849
Published
1975-02-00
Pages
333-50
Language
English
Region
England
NLM ID
0266262
PMCID
PMC1330790
Subset
IM
External Links
PubMed source DOI: 10.1113/jphysiol.1975.sp010849 PMC Full Text View journal details
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