Intrinsic and chemically produced microheterogeneity of Staphylococcus aureus enterotoxin type C.

Infection and immunity ·Vol. 12 ·No. 1 ·1975-07-00 ·Pages 93-7

Metzger JF, Johnson AD, Spero L

Abstract

Staphylococcus aureus enterotoxins C1 (SEC1) and C2 (SEC2) produced from 50-liter quantities of crude culture supernatants were purified chromatographically in a neutral or acid milieu. Microheterogenity of SEC1 was markedly increased by treatment of the purified toxin with alkali, and new more acidic charged species appeared. SEC2 was more heterogenous than any of the other S. aureus enterotoxins and was affected only slightly by treatment with alkali. Prolonged incubation of the organism during production of the SEC2 produced changes in charged species that may be related to a bacterial deamidase, since similar changes were not seen with alkaline treatment of the purified toxin. Although SEC1 and SEC2 showed complete identity immunologically, they are separate, distinct toxins, and alkali treatment of SEC1 did not produce SEC2.

MeSH Terms

Alkalies Animals Carboxymethylcellulose Sodium Densitometry Electrophoresis, Polyacrylamide Gel Enterotoxins/classification,isolation & purification Fermentation Goats/immunology Hydrogen-Ion Concentration Immune Sera Immunodiffusion Immunoelectrophoresis Isoelectric Focusing Staphylococcus/immunology

Chemicals

Alkalies Enterotoxins Immune Sera Carboxymethylcellulose Sodium

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Metzger J F

Johnson A D

Spero L

References (12)

12 references, click to expand

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Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.12.1.93-97.1975

Published

1975-07-00

Pages

93-7

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC415250

Subset

External Links

PubMed source DOI: 10.1128/iai.12.1.93-97.1975 PMC Full Text View journal details

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Intrinsic and chemically produced microheterogeneity of Staphylococcus aureus enterotoxin type C.

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