Abstract
Procaspase-8, the zymogen form of the apoptosis-initiator caspase-8, undergoes phosphorylation following integrin-mediated cell attachment to an extracellular matrix substrate. Concordant with cell attachment to fibronectin, a population of procaspase-8 becomes associated with a peripheral insoluble compartment that includes focal complexes and lamellar microfilaments. Phosphorylation of procaspase-8 both impairs its maturation to the proapoptotic form and can promote cell migration. Here we show that the cytoskeletal adaptor protein CrkL promotes caspase-8 recruitment to the peripheral spreading edge of cells, and that the catalytic domain of caspase-8 directly interacts with the SH2 domain of CrkL. We show that the interaction is abolished by shRNA-mediated silencing of Src, in Src-deficient MEFs, and by pharmacologic inhibitors of the kinase. The results provide insight into how tyrosine kinases may act to coordinate the suppression caspase-8 mediated apoptosis, while promoting cell invasion.
Keywords
Crk
Crk-L
FAK
Src
caspase 8
phosphorylation
MeSH Terms
Adaptor Proteins, Signal Transducing/genetics,metabolism
Blotting, Western
Caspase 8/genetics,metabolism
Cell Line, Tumor
Cell Movement/genetics,physiology
Enzyme-Linked Immunosorbent Assay
Fluorescent Antibody Technique
Humans
Immunohistochemistry
Nuclear Proteins/genetics,metabolism
Phosphorylation
src-Family Kinases/genetics,metabolism
Chemicals
Adaptor Proteins, Signal Transducing
CRKL protein
Nuclear Proteins
src-Family Kinases
Caspase 8
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Graf Ryon
Department of Reproductive Medicine; Division of Gynecologic Oncology; University of California San Diego School of Medicine; La Jolla, CA USA; The UCSD Moores Cancer Center; La Jolla, CA USA; Graduate School of Biomedical Sciences, Sanford-Burnham Medical Research Institute, La Jolla, CA USA.
Barbero Simone
Keller Nadine
Chen Lauren
Uryu Sean
Schlaepfer David
Stupack Dwayne
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