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题目:
Methods for the study of entosis.
作者:
Sun(Qiang),Overholtzer(Michael)
状态:
发布时间2013-06-04 , 更新时间 2013-06-04
期刊:
Methods Mol Biol
摘要:
Entosis is a recently described nonapoptotic cell death mechanism that is initiated by the engulfment of live epithelial cells, leading to the formation of "cell-in-cell" structures. Entotic cell engulfment is induced by matrix detachment, and is driven by imbalances in actomyosin contraction between neighboring cells. Here we describe methods to quantify the formation of cell-in-cell structures by entosis, for cells cultured in suspension or in soft agar, by fluorescence imaging and time-lapse microscopy.
语言:
eng
DOI:
10.1007/978-1-62703-383-1_5

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