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PMID: 2369899 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A plasminogen activator is induced during goldfish optic nerve regeneration.

The EMBO journal ·Vol. 9 ·No. 8 ·1990-08-00 ·Pages 2471-7

Sallés FJ, Schechter N, Strickland S

Abstract

The use of purified piscine plasminogen in a chromogenic solution assay enabled us to detect plasminogen activator (PA) activity in crude homogenates of goldfish optic nerve following nerve injury. In contrast, no activity was detected in the homogenates of uninjured nerve. Under conditions allowing regeneration of the optic axons (optic nerve crush), PA activity peaked 8 days after crush, and decreased to undetectable levels by 60 days. Under conditions allowing only degeneration of the axons (enucleation), the activity peaked at 8 days but decreased more rapidly. Casein zymography of samples after fractionation in SDS-PAGE showed that PA activity migrated as a doublet at Mr = 60-65 kd. Using this assay, activity was also observed in uninjured control nerves. This plasminogen-dependent activity migrated as three bands of higher molecular weight (Mr = 75, 95 and 120 kd) and was undetectable in solution assays of unfractionated extracts, suggesting complex formation with an inhibitor(s). Fibrin overlay assay of retinal explants and isolated primary cells in culture suggest that the goldfish PA is associated with the glial cells of the goldfish visual pathway.

MeSH Terms
Animals Cells, Cultured Enzyme Induction Goldfish Nerve Regeneration Optic Nerve/enzymology,physiology Organ Culture Techniques Plasminogen/isolation & purification,metabolism Plasminogen Activators/biosynthesis,metabolism Retina/enzymology
Chemicals
Plasminogen Plasminogen Activators
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sallés F J
Department of Molecular and Cellular Pharmacology, State University of New York, Stony Brook 11794.
Schechter N
Strickland S
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1990-08-00
Pages
2471-7
Language
English
Region
England
NLM ID
8208664
PMCID
PMC552275
Subset
IM
Grants
NEI NIH HHS · EY05212 · United States
NICHD NIH HHS · HD17875 · United States
NICHD NIH HHS · HD25922 · United States
External Links
PubMed source PMC Full Text View journal details
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