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PMID: 236318 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Adenosine triphosphate-induced motility and sliding of filaments in mammalian sperm extracted with Triton X-100.

The Journal of cell biology ·Vol. 65 ·No. 1 ·1975-04-00 ·Pages 147-62

Lindemann CB, Gibbons IR

Abstract

Bull sperm that had been extracted with 0.2% Triton X-100 could be reactivated with ATP, and their movement closely resembled the motion of intact live sperm. Their motility required the presence of ATP, magnesium, and a medium of suitable salt concentration and pH. When Triton-extracted bull sperm were digested breifly with trypsin at pH 9.0, they appeared to reatin most of their normal structure, but subsequent exposure of the digested sperm to ATP caused a disintegration by light microscopy, using dark-field illumination, combined with an electron microscope study of preparations of the disintegrated sperm, demonstrated the presence of an active sliding mechanism of filament interaction in bull spermatozoa. Human sperm subjected to the same procedures showed similar patterns of reactivation and of disintegration.

MeSH Terms
Adenosine Triphosphate/pharmacology Animals Cattle Cell Movement/drug effects Hydrogen-Ion Concentration Magnesium/pharmacology Male Microscopy, Electron Osmolar Concentration Polyethylene Glycols/pharmacology Quaternary Ammonium Compounds/pharmacology Spermatozoa/drug effects,physiology,ultrastructure Time Factors
Chemicals
Quaternary Ammonium Compounds Polyethylene Glycols Adenosine Triphosphate Magnesium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lindemann C B
Gibbons I R
References (34)
34 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.65.1.147
Published
1975-04-00
Pages
147-62
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2111158
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.65.1.147 PMC Full Text View journal details
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