Home LiteratureArticle Details

PMID: 2357737 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Transformation of the rice blast fungus Magnaporthe grisea to hygromycin B resistance.

Current genetics ·Vol. 17 ·No. 5 ·1990-05-00 ·Pages 409-11

Leung H, Lehtinen U, Karjalainen R, Skinner D, Tooley P, Leong S, Ellingboe A

Abstract

Low frequency, integrative transformation of three fertile hermaphroditic strains of Magnaporthe grisea has been achieved using plasmid pAN7-1 and cosmid pAN7-2, which contain an Escherichia coli hygromycin B phosphotransferase gene linked to Aspergillus nidulans regulatory sequences.

MeSH Terms

Anti-Bacterial Agents/pharmacology Ascomycota/drug effects,genetics Blotting, Southern Cosmids Drug Resistance, Microbial/genetics Hygromycin B/pharmacology Plasmids Transformation, Genetic

Chemicals

Anti-Bacterial Agents Hygromycin B

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Leung H

Department of Plant Pathology, University of Wisconsin, Madison 53706.

Lehtinen U

Karjalainen R

Skinner D

Tooley P

Leong S

Ellingboe A

References (7)

7 references, click to expand

Characterization of the Heterokaryotic and Vegetative Diploid Phases of MAGNAPORTHE GRISEA.
Genetics. 1986 Dec;114(4):1111-29 PMID: 17246357
Transformation of seven species of filamentous fungi using the nitrate reductase gene of Aspergillus nidulans.
Curr Genet. 1989 Jun;15(6):453-6 PMID: 2673557
Genetic transformation of the fungal pathogen responsible for rice blast disease.
Proc Natl Acad Sci U S A. 1987 Jun;84(12):4161-5 PMID: 16593854
Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli.
Gene. 1987;56(1):117-24 PMID: 2824287
Gene transfer system for the phytopathogenic fungus Ustilago maydis.
Proc Natl Acad Sci U S A. 1988 Feb;85(3):865-9 PMID: 2829206
Acceleration of nucleic acid hybridization rate by polyethylene glycol.
Anal Biochem. 1986 Feb 1;152(2):304-7 PMID: 2421601
Development of a fungal transformation system based on selection of sequences with promoter activity.
Mol Cell Biol. 1987 Sep;7(9):3297-305 PMID: 2823126

Article Info

Journal

Current genetics

Abbr.

Curr Genet

ISSN

0172-8083

DOI

10.1007/BF00334519

Published

1990-05-00

Pages

409-11

Language

English

Region

United States

NLM ID

8004904

Subset

External Links

PubMed source DOI: 10.1007/BF00334519 View journal details

Analysis Services

Transformation of the rice blast fungus Magnaporthe grisea to hygromycin B resistance.

Contact

WeChat Official Account