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PMID: 23558784 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A preclinical xenograft model of prostate cancer using human tumors.

Nature protocols ·Vol. 8 ·No. 5 ·2013-05-00 ·Pages 836-48

Lawrence MG, Taylor RA, Toivanen R, Pedersen J, Norden S, Pook DW, Frydenberg M, Australian Prostate Cancer BioResource, Papargiris MM, Niranjan B, Richards MG, Wang H, Collins AT, Maitland NJ, Risbridger GP

Abstract

Most cases of prostate cancer are now diagnosed as moderate-grade localized disease. These tumor specimens are important tools in the discovery and translation of prostate cancer research; however, unlike more advanced tumors, they are notoriously difficult to grow in the laboratory. We developed a system for efficiently xenografting localized human prostate cancer tissue, and we adapted this protocol to study the interactions between the specific subsets of epithelial and stromal cells. Fresh prostate tissues or isolated epithelial cells are recombined with mouse seminal vesicle mesenchyme (SVM) and grafted under the renal capsule of immunodeficient mice for optimum growth and survival. Alternatively, mouse mesenchyme can be replaced with human prostate fibroblasts in order to determine their contribution to tumor progression. Grafts can be grown for several months to determine the effectiveness of novel therapeutic compounds when administered to host mice, thereby paving the way for personalizing the treatment of individual prostate cancers.

MeSH Terms
Animals Cell Culture Techniques Cell Separation/methods Coculture Techniques Drug Evaluation, Preclinical Humans Kidney/surgery Male Mice Mice, SCID Prostatic Neoplasms/pathology Seminal Vesicles/pathology Transplantation, Heterologous/methods Xenograft Model Antitumor Assays
Authors & Affiliations
15 authors, click to expand affiliations / ORCID
Lawrence Mitchell G
Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria, Australia.
Taylor Renea A
Toivanen Roxanne
Pedersen John
Norden Sam
Pook David W
Frydenberg Mark
Australian Prostate Cancer BioResource
Papargiris Melissa M
Niranjan Birunthi
Richards Michelle G
Wang Hong
Collins Anne T
Maitland Norman J
Risbridger Gail P
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Article Info
Journal
Nature protocols
Abbr.
Nat Protoc
ISSN
1750-2799
DOI
10.1038/nprot.2013.043
Published
2013-05-00
Epub
2013-00-04
Pages
836-48
Language
English
Region
England
NLM ID
101284307
Subset
IM
Grants
Medical Research Council · G0900871 · United Kingdom
External Links
PubMed source DOI: 10.1038/nprot.2013.043 View journal details
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