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PMID: 23558772 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Control of myogenesis by rodent SINE-containing lncRNAs.

Genes & development ·Vol. 27 ·No. 7 ·2013-04-01 ·Pages 793-804

Wang J, Gong C, Maquat LE

Abstract

Staufen1-mediated mRNA decay (SMD) degrades mRNAs that harbor a Staufen1-binding site (SBS) in their 3' untranslated regions (UTRs). Human SBSs can form by intermolecular base-pairing between a 3' UTR Alu element and an Alu element within a long noncoding RNA (lncRNA) called a ½-sbsRNA. Since Alu elements are confined to primates, it was unclear how SMD occurs in rodents. Here we identify mouse mRNA 3' UTRs and lncRNAs that contain a B1, B2, B4, or identifier (ID) element. We show that SMD occurs in mouse cells via mRNA-lncRNA base-pairing of partially complementary elements and that mouse ½-sbsRNA (m½-sbsRNA)-triggered SMD regulates C2C12 cell myogenesis. Our findings define new roles for lncRNAs as well as B and ID short interspersed elements (SINEs) in mice that undoubtedly influence many developmental and homeostatic pathways.

MeSH Terms
3' Untranslated Regions/genetics Animals Base Pairing Cell Line Mice Muscle Development/genetics RNA, Long Noncoding/genetics,metabolism RNA, Messenger/genetics,metabolism RNA-Binding Proteins/metabolism Short Interspersed Nucleotide Elements/genetics Trans-Activators/metabolism
Chemicals
3' Untranslated Regions RNA, Long Noncoding RNA, Messenger RNA-Binding Proteins Rent1 protein, mouse Stau1 protein, mouse Trans-Activators
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wang Jiashi
Department of Biochemistry and Biophysics, School of Medicine and Dentistry, University of Rochester, Rochester, New York 14642, USA.
Gong Chenguang
Maquat Lynne E
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
1549-5477
DOI
10.1101/gad.212639.112
Published
2013-04-01
Epub
2013-00-04
Pages
793-804
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC3639419
Subset
IM
Grants
NIGMS NIH HHS · R01 GM074593 · United States
NIGMS NIH HHS · R37 GM074593 · United States
NIGMS NIH HHS · GM074593 · United States
External Links
PubMed source DOI: 10.1101/gad.212639.112 PMC Full Text View journal details
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