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题目:: Let-7c inhibits NSCLC cell proliferation by targeting HOXA1.
作者:: Zhan(Min),Qu(Qiang),Wang(Guo),Liu(Ying-Zi),Tan(Sheng-Lan),Lou(Xiao-Ya),Yu(Jing),Zhou(Hong-Hao)
状态:: 发布时间2013-03-28 , 更新时间 2013-03-28
期刊:: Asian Pac J Cancer Prev
摘要:: The aim of the present study was to explore mechanisms by which let-7c suppresses NSCLC cell proliferation.,The expression level of let-7c was quantified by qRT-PCR. A549 and H1299 cells were transfected with let-7c mimics to restore the expression of let-7c. The effects of let-7c were then assessed by cell proliferation, colony formation and cell cycle assay. Mouse experiments were used to confirm the effect of let-7c on tumorigenicity in vivo. Luciferase reporter assays and Western blotting were performed to identify target genes for let-7c.,HOXA1 was identified as a novel target of let-7c. MTS, colony formation and flow cytometry assays demonstrated that forced expression of let-7c inhibited NSCLC cell proliferation by inducing G1 arrest in vitro, consistent with inhibitory effects induced by knockdown of HOXA1. Mouse experiments demonstrated that let-7c expression suppressed tumorigenesis. Furthermore, we found that let-7c could regulate the expression of HOXA1 downstream effectors CCND1, CDC25A and CDK2.,Collectively, these results demonstrate let-7c inhibits NSCLC cell proliferation and tumorigenesis by partial direct targeting of the HOXA1 pathway, which suggests that restoration of let-7c expression may thus offer a potential therapeutic intervention strategy for NSCLC.
语言:: eng
DOI:

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