Deterioration induced by physiological concentration of calcium ions in skinned muscle fibres.

Journal of muscle research and cell motility ·Vol. 11 ·No. 1 ·1990-02-00 ·Pages 41-7

Kasuga N, Umazume Y

Abstract

The deteriorating effect of microM order of Ca2+ on skinned frog skeletal muscle fibres was studied from the view point of the digestion of proteins by calcium-activated neutral protease (CANP). Tension developed in solutions containing no MgATP (rigor solution) decreased irreversibly with the addition of Ca2+ in quantities of more than 0.1 microM. Low temperature was seen to suppress (Q10 greater than 4), and neutral pH to maximize, this decrease in tension. In rigor solution containing Ca2+, SDS electrophoresis indicated that a 95 k dalton component (alpha-actinin) was released from the fibre; electron micrography showed the disappearance of Z-lines. These results suggest that one of the causes for decrease in rigor tension is the proteolytic activity of CANP, and its inhibitors were shown to be quite useful in experiments on skinned fibre.

MeSH Terms

Animals Antipain/pharmacology Calcium/pharmacology Cysteine Proteinase Inhibitors/pharmacology Dose-Response Relationship, Drug Electrophoresis, Polyacrylamide Gel Hydrogen-Ion Concentration Iodoacetates/pharmacology Iodoacetic Acid Leucine/analogs & derivatives,pharmacology Leupeptins/pharmacology Microscopy, Electron Muscle Contraction/drug effects,physiology Muscles/drug effects,physiopathology,ultrastructure Rana catesbeiana Temperature

Chemicals

Cysteine Proteinase Inhibitors Iodoacetates Leupeptins Antipain Leucine E 64 Calcium Iodoacetic Acid

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Kasuga N

Department of Physiology, Jikei University School of Medicine, Tokyo, Japan.

Umazume Y

References (21)

21 references, click to expand

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Article Info

Journal

Journal of muscle research and cell motility

Abbr.

J Muscle Res Cell Motil

ISSN

0142-4319

DOI

10.1007/BF01833324

Published

1990-02-00

Pages

41-7

Language

English

Region

Netherlands

NLM ID

8006298

Subset

External Links

PubMed source DOI: 10.1007/BF01833324 View journal details

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Deterioration induced by physiological concentration of calcium ions in skinned muscle fibres.

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