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PMID: 23511481 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Arabidopsis NIN-like transcription factors have a central role in nitrate signalling.

Nature communications ·Vol. 4 ·2013-00-00 ·Pages 1617

Konishi M, Yanagisawa S

Abstract

In plants, nitrate is not only a major nitrogen source but also a signalling molecule that modulates the expression of a wide range of genes and that regulates growth and development. The critical role of nitrate as a signalling molecule has been established for several decades. However, the molecular mechanisms underlying the nitrate response have remained elusive, as the transcription factor that primarily responds to nitrate signals has not yet been identified. Here we show that Arabidopsis NIN-LIKE PROTEIN (NLP) family proteins bind the nitrate-responsive cis-element and activate nitrate-responsive cis-element-dependent and nitrate-responsive transcription. Our results also suggest that the activity of NLPs is post-translationally modulated by nitrate signalling. Furthermore, the suppression of NLP function impairs the nitrate-inducible expression of a number of genes and causes severe growth inhibition. These results indicate that NLPs are the transcription factors mediating the nitrate signal and thereby function as master regulators of the nitrate response.

MeSH Terms
Arabidopsis/genetics,physiology Arabidopsis Proteins/physiology Electrophoretic Mobility Shift Assay Genes, Plant Nitrates/metabolism Plants, Genetically Modified Reverse Transcriptase Polymerase Chain Reaction Signal Transduction/physiology Transcription Factors/physiology Transcription, Genetic
Chemicals
Arabidopsis Proteins Nitrates Transcription Factors
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Konishi Mineko
Laboratory of Plant Biotechnology, Biotechnology Research Center, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
Yanagisawa Shuichi
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Article Info
Journal
Nature communications
Abbr.
Nat Commun
ISSN
2041-1723
DOI
10.1038/ncomms2621
Published
2013-00-00
Pages
1617
Language
English
Region
England
NLM ID
101528555
Subset
IM
External Links
PubMed source DOI: 10.1038/ncomms2621 View journal details
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