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PMID: 23487420 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Conversion of peripheral blood NK cells to a decidual NK-like phenotype by a cocktail of defined factors.

Journal of immunology (Baltimore, Md. : 1950) ·Vol. 190 ·No. 8 ·2013-04-15 ·Pages 3939-48

Cerdeira AS, Rajakumar A, Royle CM, Lo A, Husain Z, Thadhani RI, Sukhatme VP, Karumanchi SA, Kopcow HD

Abstract

NK cells that populate the decidua are important regulators of normal placentation. In contrast to peripheral blood NK cells, decidual NK (dNK) cells lack cytotoxicity, secrete proangiogenic factors, and regulate trophoblast invasion. In this study we show that exposure to a combination of hypoxia, TGF-β1, and a demethylating agent results in NK cells that express killer cell Ig-like receptors, the dNK cell markers CD9 and CD49a, and a dNK pattern of chemokine receptors. These cells secrete vascular endothelial growth factor (a potent proangiogenic molecule), display reduced cytotoxicity, and promote invasion of human trophoblast cell lines. These findings have potential therapeutic applications for placental disorders associated with altered NK cell biology.

MeSH Terms
Angiogenic Proteins/biosynthesis,blood,physiology Azacitidine/analogs & derivatives,pharmacology CD56 Antigen/biosynthesis,blood,physiology Cell Line, Transformed Cell Movement/immunology Cytoplasmic Granules/immunology Decidua/cytology,immunology,metabolism Decitabine Female GPI-Linked Proteins/biosynthesis,blood,physiology Human Umbilical Vein Endothelial Cells Humans Immunophenotyping Killer Cells, Natural/immunology,metabolism Receptors, IgG/biosynthesis,blood,physiology Receptors, KIR/biosynthesis,blood,physiology
Chemicals
Angiogenic Proteins CD56 Antigen FCGR3B protein, human GPI-Linked Proteins NCAM1 protein, human Receptors, IgG Receptors, KIR Decitabine Azacitidine
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Cerdeira Ana Sofia
Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
Rajakumar Augustine
Royle Caroline M
Lo Agnes
Husain Zaheed
Thadhani Ravi I
Sukhatme Vikas P
Karumanchi S Ananth
Kopcow Hernan D
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Article Info
Journal
Journal of immunology (Baltimore, Md. : 1950)
Abbr.
J Immunol
ISSN
1550-6606
DOI
10.4049/jimmunol.1202582
Published
2013-04-15
Epub
2013-00-13
Pages
3939-48
Language
English
Region
United States
NLM ID
2985117R
PMCID
PMC3742368
Subset
IM
Grants
NICHD NIH HHS · K12 HD051959 · United States
Howard Hughes Medical Institute · United States
External Links
PubMed source DOI: 10.4049/jimmunol.1202582 PMC Full Text View journal details
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