Powdered chitin agar as a selective medium for enumeration of actinomycetes in water and soil.

Applied microbiology ·Vol. 29 ·No. 3 ·1975-03-00 ·Pages 422-6

Hsu SC, Lockwood JL

Abstract

Agar media made with 0.4% colloidal chitin plus mineral salts and adjusted to pH 8.0 was superior to four other commonly used media for the isolation and enumeration of actinomycetes from water samples. More actinomycetes developed on chitin agar, and the development of bacteria and fungi was suppressed. Frozen and vacuum-dried chitin from aqueous colloidal suspensions was finely divided and gave results comparable to those obtained with media prepared from colloidal suspensions.

MeSH Terms

Actinomycetales/isolation & purification Agar Arginine Caseins Chitin Colloids Evaluation Studies as Topic Glycerol Hydrogen-Ion Concentration Micromonospora/isolation & purification Minerals Nocardia/isolation & purification Ovalbumin Powders Salts Soil Microbiology Starch Streptomyces/isolation & purification Water Microbiology

Chemicals

Caseins Colloids Minerals Powders Salts Chitin Agar Starch Ovalbumin Arginine Glycerol

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Hsu S C

Lockwood J L

References (10)

10 references, click to expand

The selective enumeration of Actinomycetes in the presence of large numbers of fungi.
Can J Microbiol. 1956 Feb;2(1):12-6 PMID: 13293580
[Streptomyces production of exochitinase].
C R Seances Soc Biol Fil. 1955 Jun;149(11-12):1307-8 PMID: 13277305
Control of mold contaminants on solid media by the use of actidione.
J Bacteriol. 1950 Jul;60(1):104-5 PMID: 15436468
SELECTION OF MEDIA FOR ISOLATION OF STREPTOMYCETES.
Nature. 1964 May 30;202:928-9 PMID: 14190108
Lysis of fungal mycelium by Streptomyces spp. and their chitinase systems.
Phytopathology. 1965 Aug;55(8):871-5 PMID: 5827065
A chitin medium for isolation, growth and maintenance of actinomycetes.
Nature. 1961 Jan 14;189:158-9 PMID: 13762423
Exocellular chitinase from a Streptomyces sp.
J Gen Microbiol. 1954 Oct;11(2):150-9 PMID: 13211969
The use of sodium propionate in isolating actinomycetes from soils.
Science. 1950 Dec 1;112(2918):656 PMID: 14787500
[Use of a medium containing chitin for isolation and counting actinomycetes from soil].
Mikrobiologiia. 1970 Sep-Oct;39(5):902-6 PMID: 5497286
Selective isolation of aerobic Actinomycetes.
Appl Microbiol. 1963 Mar;11:75-7 PMID: 13937509

Article Info

Journal

Applied microbiology

Abbr.

Appl Microbiol

ISSN

0003-6919

DOI

10.1128/am.29.3.422-426.1975

Published

1975-03-00

Pages

422-6

Language

English

Region

United States

NLM ID

7605802

PMCID

PMC186990

Subset

External Links

PubMed source DOI: 10.1128/am.29.3.422-426.1975 PMC Full Text

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Powdered chitin agar as a selective medium for enumeration of actinomycetes in water and soil.

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