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PMID: 2345139 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Manganese regulates expression of manganese peroxidase by Phanerochaete chrysosporium.

Journal of bacteriology ·Vol. 172 ·No. 6 ·1990-06-00 ·Pages 3125-30

Brown JA, Glenn JK, Gold MH

Abstract

The appearance of manganese peroxidase (MnP) activity in nitrogen-limited cultures of Phanerochaete chrysosporium is dependent on the presence of manganese. Cultures grown in the absence of Mn developed normally and produced normal levels of the secondary metabolite veratryl alcohol but produced no MnP activity. Immunoblot analysis indicated that appearance of MnP protein in the extracellular medium was also dependent on the presence of Mn. Intracellular MnP protein was detectable only in cells grown in the presence of Mn. MnP mRNA was detected by Northern (RNA) blot analysis only in cells grown in the presence of Mn. If Mn was added to 4-day-old nitrogen-limited Mn-deficient cultures, extracellular MnP activity appeared after 6 h and reached a maximum after 18 h. Both actinomycin D and cycloheximide inhibited the induction of MnP activity by Mn. These results indicate that Mn, the substrate of the enzyme, is involved in the transcriptional regulation of the MnP gene.

MeSH Terms
Basidiomycota/enzymology Dactinomycin/pharmacology Dose-Response Relationship, Drug Enzyme Induction Gene Expression Regulation, Enzymologic Lignin/metabolism Manganese/pharmacology Peroxidases/biosynthesis
Chemicals
Dactinomycin Manganese Lignin Peroxidases lignin peroxidase manganese peroxidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Brown J A
Department of Chemical and Biological Sciences, Oregon Graduate Institute of Science and Technology, Beaverton 97006-1999.
Glenn J K
Gold M H
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.172.6.3125-3130.1990
Published
1990-06-00
Pages
3125-30
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC209116
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.172.6.3125-3130.1990 PMC Full Text View journal details
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