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PMID: 23446052 Published · epublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Review

The role of the nucleosome acidic patch in modulating higher order chromatin structure.

Journal of the Royal Society, Interface ·Vol. 10 ·No. 82 ·2013-05-06 ·Pages 20121022

Kalashnikova AA, Porter-Goff ME, Muthurajan UM, Luger K, Hansen JC

Abstract

Higher order folding of chromatin fibre is mediated by interactions of the histone H4 N-terminal tail domains with neighbouring nucleosomes. Mechanistically, the H4 tails of one nucleosome bind to the acidic patch region on the surface of adjacent nucleosomes, causing fibre compaction. The functionality of the chromatin fibre can be modified by proteins that interact with the nucleosome. The co-structures of five different proteins with the nucleosome (LANA, IL-33, RCC1, Sir3 and HMGN2) recently have been examined by experimental and computational studies. Interestingly, each of these proteins displays steric, ionic and hydrogen bond complementarity with the acidic patch, and therefore will compete with each other for binding to the nucleosome. We first review the molecular details of each interface, focusing on the key non-covalent interactions that stabilize the protein-acidic patch interactions. We then propose a model in which binding of proteins to the nucleosome disrupts interaction of the H4 tail domains with the acidic patch, preventing the intrinsic chromatin folding pathway and leading to assembly of alternative higher order chromatin structures with unique biological functions.

MeSH Terms
Animals Chromatin Assembly and Disassembly/physiology Chromosomal Proteins, Non-Histone/chemistry,genetics,metabolism Humans Models, Molecular Nucleosomes/chemistry,genetics,metabolism
Chemicals
Chromosomal Proteins, Non-Histone Nucleosomes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kalashnikova Anna A
Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523-1870, USA.
Porter-Goff Mary E
Muthurajan Uma M
Luger Karolin
Hansen Jeffrey C
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Article Info
Journal
Journal of the Royal Society, Interface
Abbr.
J R Soc Interface
ISSN
1742-5662
DOI
10.1098/rsif.2012.1022
Published
2013-05-06
Epub
2013-00-27
Pages
20121022
Language
English
Region
England
NLM ID
101217269
PMCID
PMC3627075
Subset
IM
Grants
Howard Hughes Medical Institute · United States
NIGMS NIH HHS · GM066834 · United States
NIGMS NIH HHS · P01 GM088409 · United States
NIGMS NIH HHS · GM088409 · United States
NIGMS NIH HHS · R01 GM045916 · United States
NIGMS NIH HHS · R01 GM066834 · United States
NIGMS NIH HHS · GM045916 · United States
External Links
PubMed source DOI: 10.1098/rsif.2012.1022 PMC Full Text View journal details
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