| GeneLibs

题目:: Regeneration of human tumor antigen-specific T cells from iPSCs derived from mature CD8(+) T cells.
作者:: Vizcardo(Raul),Masuda(Kyoko),Yamada(Daisuke),Ikawa(Tomokatsu),Shimizu(Kanako),Fujii(Shin-Ichiro),Koseki(Haruhiko),Kawamoto(Hiroshi)
状态:: 发布时间2013-01-07 , 更新时间 2016-05-18
期刊:: Cell Stem Cell
摘要:: Antigen-specific T cells represent a potential therapeutic avenue for a variety of conditions, but current approaches for generating such cells for therapeutic purposes are limited. In this study, we established iPSCs from mature cytotoxic T cells specific for the melanoma epitope MART-1. When cocultured with OP9/DLL1 cells, these iPSCs efficiently generated TCRβ(+)CD4(+)CD8(+) double positive (DP) cells expressing a T cell receptor (TCR) specific for the MART-1 epitope. Stimulation of these DP cells with anti-CD3 antibody generated a large number of CD8(+) T cells, and more than 90% of the resulting cells were specific for the original MART-1 epitope. Stimulation of the CD8(+) T cells with MART-1 antigen-presenting cells led to the secretion of IFNγ, demonstrating their specific reactivity. The present study therefore illustrates an approach for cloning and expanding functional antigen-specific CD8(+) T cells that might be applicable in cell-based therapy of cancer.
语言:: eng
DOI:: 10.1016/j.stem.2012.12.006

文献库文献相关信息