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PMID: 2325657 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Identification of mitogen-responsive ribosomal protein S6 kinase pp90rsk, a homolog of Xenopus S6 kinase II, in chicken embryo fibroblasts.

Molecular and cellular biology ·Vol. 10 ·No. 5 ·1990-05-00 ·Pages 2413-7

Sweet LJ, Alcorta DA, Jones SW, Erikson E, Erikson RL

Abstract

Antiserum raised against recombinant Xenopus ribosomal protein S6 kinase (rsk) was used to identify a 90,000-Mr ribosomal S6 kinase, pp90rsk, in chicken embryo fibroblasts. Adding serum to cells stimulated the phosphorylation of pp90rsk on serine and threonine residues and increased the activity of S6 kinase measured in immune complex assays. Xenopus S6 kinase II and chicken embryo fibroblast pp90rsk had nearly identical phosphopeptide maps.

MeSH Terms

Animals Blotting, Western Chick Embryo Molecular Weight Peptide Mapping Phosphoproteins/analysis,metabolism Precipitin Tests Protein Kinases/analysis Ribosomal Protein S6 Kinases

Chemicals

Phosphoproteins Protein Kinases Ribosomal Protein S6 Kinases

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Sweet L J

Department of Cellular and Developmental Biology, Harvard University, Cambridge, Massachusetts 02138.

Alcorta D A

Jones S W

Erikson E

Erikson R L

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26 references, click to expand

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Article Info

Journal

Molecular and cellular biology

Abbr.

Mol Cell Biol

ISSN

0270-7306

DOI

10.1128/mcb.10.5.2413-2417.1990

Published

1990-05-00

Pages

2413-7

Language

English

Region

United States

NLM ID

8109087

PMCID

PMC360590

Subset

Grants

NCI NIH HHS · CA42580 · United States

External Links

PubMed source DOI: 10.1128/mcb.10.5.2413-2417.1990 PMC Full Text View journal details

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Identification of mitogen-responsive ribosomal protein S6 kinase pp90rsk, a homolog of Xenopus S6 kinase II, in chicken embryo fibroblasts.

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