| GeneLibs

题目:: Expression analysis of mir-17-5p, mir-20a and let-7a microRNAs and their target proteins in CD34+ bone marrow cells of patients with myelodysplastic syndromes.
作者:: Vasilatou(Diamantina),Papageorgiou(Sotirios G),Kontsioti(Frieda),Kontos(Christos K),Tsiotra(Panayiota),Mpakou(Vassiliki),Pavlou(Maria-Angeliki S),Economopoulou(Christina),Dimitriadis(George),Dervenoulas(John),Pappa(Vasiliki)
状态:: 发布时间2013-01-29 , 更新时间 2013-01-29
期刊:: Leuk Res
摘要:: Mir-17-5p and mir-20a, members of the mir-17-92 family, down-regulate E2F1, which is over-expressed in myelodysplastic syndromes (MDS). Moreover, let-7a down-regulates KRAS, which is aberrantly expressed in MDS. We evaluated the expression of the aforementioned microRNAs in CD34+ cells of 43 MDS patients using real-time PCR and their target proteins (E2F1, MYC, BCL2, CCND1, and KRAS) by Western blot. Mir-17-5p and mir-20a were under expressed in high risk MDS patients, compared to low risk MDS patients. Similarly, let-7a was under expressed in patients with intermediate or high-risk karyotype. Interestingly, there was an inverse correlation between microRNA and the expression levels of their targets. Importantly, mir-17-5p and mir-20a constitute favorable prognostic factors in MDS, since their expression was associated with increased overall survival of MDS patients.
语言:: eng
DOI:: 10.1016/j.leukres.2012.11.011

文献库文献相关信息