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PMID: 23241948 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Circadian clock adjustment to plant iron status depends on chloroplast and phytochrome function.

The EMBO journal ·Vol. 32 ·No. 4 ·2013-02-20 ·Pages 511-23

Salomé PA, Oliva M, Weigel D, Krämer U

Abstract

Plant chloroplasts are not only the main cellular location for storage of elemental iron (Fe), but also the main site for Fe, which is incorporated into chlorophyll, haem and the photosynthetic machinery. How plants measure internal Fe levels is unknown. We describe here a new Fe-dependent response, a change in the period of the circadian clock. In Arabidopsis, the period lengthens when Fe becomes limiting, and gradually shortens as external Fe levels increase. Etiolated seedlings or light-grown plants treated with plastid translation inhibitors do not respond to changes in Fe supply, pointing to developed chloroplasts as central hubs for circadian Fe sensing. Phytochrome-deficient mutants maintain a short period even under Fe deficiency, stressing the role of early light signalling in coupling the clock to Fe responses. Further mutant and pharmacological analyses suggest that known players in plastid-to-nucleus signalling do not directly participate in Fe sensing. We propose that the sensor governing circadian Fe responses defines a new retrograde pathway that involves a plastid-encoded protein that depends on phytochromes and the functional state of chloroplasts.

MeSH Terms
Arabidopsis/genetics,metabolism Cell Nucleus/genetics,metabolism Chloroplasts/genetics,metabolism Circadian Clocks/physiology Iron/metabolism Mutation Phytochrome/genetics,metabolism Seedlings/genetics,metabolism Signal Transduction/physiology
Chemicals
Phytochrome Iron
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Salomé Patrice A
Department of Molecular Biology, Max Planck Institute for Developmental Biology, Tübingen, Germany. patrice.salome@tuebingen.mpg.de
Oliva Michele
Weigel Detlef
Krämer Ute
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
1460-2075
DOI
10.1038/emboj.2012.330
Email
patrice.salome@tuebingen.mpg.de
Published
2013-02-20
Epub
2012-00-14
Pages
511-23
Language
English
Region
England
NLM ID
8208664
PMCID
PMC3579136
Subset
IM
Corrections
CommentIn
PMID: 23376920
External Links
PubMed source DOI: 10.1038/emboj.2012.330 PMC Full Text View journal details
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