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PMID: 2318968 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Adaptation to bacterial lipopolysaccharide controls lipopolysaccharide-induced tumor necrosis factor production in rabbit macrophages.

The Journal of clinical investigation ·Vol. 85 ·No. 4 ·1990-04-00 ·Pages 1108-18

Mathison JC, Virca GD, Wolfson E, Tobias PS, Glaser K, Ulevitch RJ

Abstract

These experiments provide an explanation for the observation that two intravenous injections of lipopolysaccharide (LPS) spaced 5 h apart in rabbits cause tumor necrosis factor/cachectin (TNF) levels to rise in the blood only after the first LPS injection. Herein we show that treatment of elicited peritoneal exudate rabbit macrophages (PEM) with two doses of LPS given 9 h apart results in a marked reduction in TNF production by the second LPS exposure. This state of hyporesponsiveness is a result of adaptation to LPS, is induced by LPS concentrations that are 1,000-fold less than required to induce TNF production (picograms vs. nanograms), is characterized by a decrease in LPS-induced TNF mRNA without any change in TNF mRNA half-life, is not changed by including indomethacin in cultures, and is specific for LPS since LPS-adapted cells display a TNF response to heat-killed Staphylococcus aureus that is at least as good as that observed in control PEM.

MeSH Terms
Adaptation, Physiological/drug effects Animals Dose-Response Relationship, Drug Indomethacin/pharmacology Interleukin-1/biosynthesis Lipopolysaccharides/metabolism,pharmacology Macrophages/metabolism RNA, Messenger/analysis Rabbits Staphylococcus aureus/physiology Stereoisomerism Tumor Necrosis Factor-alpha/biosynthesis,genetics
Chemicals
Interleukin-1 Lipopolysaccharides RNA, Messenger Tumor Necrosis Factor-alpha Indomethacin
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Mathison J C
Department of Immunology, Research Institute of Scripps Clinic, La Jolla, California 92037.
Virca G D
Wolfson E
Tobias P S
Glaser K
Ulevitch R J
References (40)
40 references, click to expand
  1. Synergy between tumor necrosis factor and bacterial products causes hemorrhagic necrosis and lethal shock in normal mice.
    Proc Natl Acad Sci U S A. 1988 Jan;85(2):607-11 PMID: 3422444
  2. Anti-cachectin/TNF monoclonal antibodies prevent septic shock during lethal bacteraemia.
    Nature. 1987 Dec 17-23;330(6149):662-4 PMID: 3317066
  3. Prostaglandin E2 regulates macrophage-derived tumor necrosis factor gene expression.
    J Biol Chem. 1988 Apr 15;263(11):5380-4 PMID: 3162731
  4. Regulation of tumor necrosis factor/cachectin and IL-1 secretion in human mononuclear phagocytes.
    J Immunol. 1988 May 15;140(10):3473-81 PMID: 3258884
  5. Transcriptional and posttranscriptional regulation of tumor necrosis factor gene expression in human monocytes.
    J Clin Invest. 1988 May;81(5):1506-10 PMID: 3366904
  6. Assay of a ribonuclease that preferentially hydrolyses mRNAs containing cytokine-derived UA-rich instability sequences.
    Biochem Biophys Res Commun. 1988 May 16;152(3):973-80 PMID: 3288201
  7. Participation of tumor necrosis factor in the mediation of gram negative bacterial lipopolysaccharide-induced injury in rabbits.
    J Clin Invest. 1988 Jun;81(6):1925-37 PMID: 3384955
  8. Specific endotoxic lipopolysaccharide-binding proteins on murine splenocytes. I. Detection of lipopolysaccharide-binding sites on splenocytes and splenocyte subpopulations.
    J Immunol. 1988 Aug 1;141(3):996-1005 PMID: 2456339
  9. Tolerance to tumor necrosis factor in rats and the relationship to endotoxin tolerance and toxicity.
    J Exp Med. 1988 Jul 1;168(1):95-105 PMID: 3294337
  10. Lipid A binding sites in membranes of macrophage tumor cells.
    J Biol Chem. 1988 Oct 15;263(29):14802-7 PMID: 3170565
  11. Cachectin/tumor necrosis factor exerts endocrine, paracrine, and autocrine control of inflammatory responses.
    J Cell Biol. 1988 Oct;107(4):1269-77 PMID: 3049617
  12. Release of tumor necrosis factor-alpha from macrophages. Enhancement and suppression are dose-dependently regulated by prostaglandin E2 and cyclic nucleotides.
    J Immunol. 1988 Oct 1;141(7):2388-93 PMID: 2844899
  13. Reduced release of TNF and PCA from macrophages of tolerant mice.
    Circ Shock. 1988 Oct;26(2):185-92 PMID: 3197264
  14. Macrophages rapidly internalize their tumor necrosis factor receptors in response to bacterial lipopolysaccharide.
    J Biol Chem. 1989 Mar 5;264(7):3924-9 PMID: 2537297
  15. IL-1 induction-capacity of defined lipopolysaccharide partial structures.
    J Immunol. 1989 May 1;142(9):3229-38 PMID: 2651523
  16. Inhibition studies on the membrane-associated phospholipase A2 in vitro and prostaglandin E2 production in vivo of the macrophage-like P388D1 cell. Effects of manoalide, 7,7-dimethyl-5,8-eicosadienoic acid, and p-bromophenacyl bromide.
    J Biol Chem. 1989 May 25;264(15):8520-8 PMID: 2498315
  17. Modulation of tumor necrosis factor-alpha gene expression. Desensitization of prostaglandin E2-induced suppression.
    J Immunol. 1989 Jun 15;142(12):4346-50 PMID: 2723432
  18. Optimal induction of tumor necrosis factor production in human monocytes requires complete S-form lipopolysaccharide.
    Infect Immun. 1989 Jul;57(7):1953-8 PMID: 2731978
  19. In vivo induction of IL-6 by administration of exogenous cytokines and detection of de novo serum levels of IL-6 in tumor-bearing mice.
    J Immunol. 1989 Jul 1;143(1):162-7 PMID: 2499626
  20. Downregulation of tumor necrosis factor expression in the human Mono-Mac-6 cell line by lipopolysaccharide.
    J Leukoc Biol. 1989 Jul;46(1):11-4 PMID: 2732625
  21. Production of human tumor necrosis factor from whole blood ex vivo.
    Lymphokine Res. 1989 Summer;8(2):141-6 PMID: 2659900
  22. Lipopolysaccharide induces hyporesponsiveness to its own action in RAW 264.7 cells.
    J Biol Chem. 1989 Dec 25;264(36):21951-6 PMID: 2480960
  23. Membrane properties of living mammalian cells as studied by enzymatic hydrolysis of fluorogenic esters.
    Proc Natl Acad Sci U S A. 1966 Jan;55(1):134-41 PMID: 5220862
  24. A new method for the extraction of R lipopolysaccharides.
    Eur J Biochem. 1969 Jun;9(2):245-9 PMID: 5804498
  25. Ribonucleic acid isolated by cesium chloride centrifugation.
    Biochemistry. 1974 Jun 4;13(12):2633-7 PMID: 4831907
  26. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
    Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
  27. The analysis of nucleic acids in gels using glyoxal and acridine orange.
    Methods Enzymol. 1980;65(1):380-91 PMID: 6154866
  28. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  29. Cultured human monocytes require exposure to bacterial products to maintain an optimal oxygen radical response.
    J Immunol. 1982 Jan;128(1):123-8 PMID: 6274954
  30. Amplification and adaptation in regulatory and sensory systems.
    Science. 1982 Jul 16;217(4556):220-5 PMID: 7089556
  31. A new troponin T and cDNA clones for 13 different muscle proteins, found by shotgun sequencing.
    Nature. 1983 Apr 21;302(5910):718-21 PMID: 6687628
  32. Interaction between endotoxin and human monocytes: characteristics of the binding of 3H-labeled lipopolysaccharide and 51Cr-labeled lipid A before and after the induction of endotoxin tolerance.
    Proc Natl Acad Sci U S A. 1984 Jun;81(11):3491-5 PMID: 6587364
  33. Prostaglandins as endogenous mediators of interleukin 1 production.
    J Immunol. 1986 Jan;136(1):186-92 PMID: 3079606
  34. Control of cachectin (tumor necrosis factor) synthesis: mechanisms of endotoxin resistance.
    Science. 1986 May 23;232(4753):977-80 PMID: 3754653
  35. Molecular cloning and expression in Escherichia coli of the cDNA coding for rabbit tumor necrosis factor.
    DNA. 1986 Apr;5(2):149-56 PMID: 3519137
  36. A conserved AU sequence from the 3' untranslated region of GM-CSF mRNA mediates selective mRNA degradation.
    Cell. 1986 Aug 29;46(5):659-67 PMID: 3488815
  37. Prostaglandins posttranscriptionally inhibit monocyte expression of interleukin 1 activity by increasing intracellular cyclic adenosine monophosphate.
    J Immunol. 1986 Nov 15;137(10):3189-94 PMID: 3021848
  38. Effect of gamma interferon on cachectin expression by mononuclear phagocytes. Reversal of the lpsd (endotoxin resistance) phenotype.
    J Exp Med. 1986 Nov 1;164(5):1791-6 PMID: 3095481
  39. Trace levels of bacterial lipopolysaccharide prevent interferon-gamma or tumor necrosis factor-alpha from enhancing mouse peritoneal macrophage respiratory burst capacity.
    J Immunol. 1987 Sep 15;139(6):1971-7 PMID: 3040860
  40. Induction of tolerance to lipopolysaccharide (LPS)-D-galactosamine lethality by pretreatment with LPS is mediated by macrophages.
    Infect Immun. 1988 May;56(5):1352-7 PMID: 3356468
Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI114542
Published
1990-04-00
Pages
1108-18
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC296541
Subset
IM
Grants
NIAID NIH HHS · AI-15136 · United States
NIAID NIH HHS · AI-25563 · United States
NIGMS NIH HHS · GM-28485 · United States
External Links
PubMed source DOI: 10.1172/JCI114542 PMC Full Text View journal details
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