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PMID: 23185391 Published · ppublish English Comparative Study Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

A comparative study of the Arabidopsis thaliana guard-cell transcriptome and its modulation by sucrose.

PloS one ·Vol. 7 ·No. 11 ·2012-00-00 ·Pages e49641

Bates GW, Rosenthal DM, Sun J, Chattopadhyay M, Peffer E, Yang J, Ort DR, Jones AM

Abstract

Microarray analysis was performed on RNA isolated from guard cells that were manually dissected from leaves of Arabidopsis. By pooling our data with those of two earlier studies on Arabidopsis guard cell protoplasts, we provide a robust view of the guard-cell transcriptome, which is rich in transcripts for transcription factors, signaling proteins, transporters, and carbohydrate-modifying enzymes. To test the hypothesis that photosynthesis-derived sugar signals guard cells to adjust stomatal opening, we determined the profile of genes expressed in guard cells from leaves that had been treated with sucrose. The results revealed that expression of 440 genes changed in guard cells in response to sucrose. Consistent with this hypothesis, these genes encoded cellular functions for photosynthesis and transport of sugars, water, amino acids, and ions. Plants of T-DNA insertion lines for 50 genes highly responsive to sucrose were examined for defects in guard cell function. Twelve genes not previously known to function in guard cells were shown to be important in leaf conductance, water-use efficiency, and/or stomate development. Of these, three are of particular interest, having shown effects in nearly every test of stomatal function without a change in stomatal density: TPS5 (At4g17770), a TRAF domain-containing protein (At1g65370), and a WD repeat-containing protein (At1g15440).

MeSH Terms
Arabidopsis/genetics,metabolism Arabidopsis Proteins/genetics Computational Biology/methods DNA, Bacterial Gene Expression Regulation, Plant Genes, Plant Oligonucleotide Array Sequence Analysis Plant Leaves/metabolism Plant Stomata/metabolism Real-Time Polymerase Chain Reaction/methods Signal Transduction Sucrose/chemistry Transcriptome
Chemicals
Arabidopsis Proteins DNA, Bacterial T-DNA Sucrose
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Bates George W
Department of Biological Science, Florida State University, Tallahassee, Florida, United States of America. bates@bio.fsu.edu
Rosenthal David M
Sun Jindong
Chattopadhyay Maitreyi
Peffer Emily
Yang Jing
Ort Donald R
Jones Alan M
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Article Info
Journal
PloS one
Abbr.
PLoS One
ISSN
1932-6203
DOI
10.1371/journal.pone.0049641
Email
bates@bio.fsu.edu
Published
2012-00-00
Epub
2012-00-21
Pages
e49641
Language
English
Region
United States
NLM ID
101285081
PMCID
PMC3504121
Subset
IM
Grants
NIGMS NIH HHS · R01 GM065989 · United States
NIGMS NIH HHS · R01GM065989 · United States
External Links
PubMed source DOI: 10.1371/journal.pone.0049641 PMC Full Text View journal details
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