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PMID: 2315308 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Isolation and characterization of a cDNA clone encoding avian skeletal muscle C-protein: an intracellular member of the immunoglobulin superfamily.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 6 ·1990-03-00 ·Pages 2157-61

Einheber S, Fischman DA

Abstract

C-protein is a thick filament-associated protein located in the crossbridge region of vertebrate striated muscle A bands. Its function is unknown. To improve our understanding of its primary structure, we undertook the molecular cloning of C-protein mRNA. We describe the isolation and characterization of a cDNA clone, lambda C-86, that encodes approximately 80% of the fast isoform of C-protein in the chicken. Sequence analysis of the insert revealed that C-protein, although an intracellular, nonmembrane-associated protein, is a member of the immunoglobulin superfamily. Like several cell surface adhesion molecules that belong to this superfamily, C-protein contains sequence motifs that resemble immunoglobulin domains and fibronectin type III repeats. Computer searches using the C-protein sequence also lead to the identification of related domains in chicken smooth muscle myosin light chain kinase that have not been reported previously.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Blotting, Northern Carrier Proteins Chickens Cloning, Molecular DNA/genetics,isolation & purification Gene Library Genes, Immunoglobulin Humans Molecular Sequence Data Multigene Family Muscle Proteins/genetics Muscles/metabolism Protein Biosynthesis RNA, Messenger/genetics,isolation & purification Sequence Homology, Nucleic Acid
Chemicals
Carrier Proteins Muscle Proteins RNA, Messenger myosin-binding protein C DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Einheber S
Department of Cell Biology and Anatomy, Cornell University Medical College, New York, NY 10021.
Fischman D A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.6.2157
Published
1990-03-00
Pages
2157-61
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC53645
Subset
IM
Grants
NIAMS NIH HHS · AR32147 · United States
Databases
GENBANK
M31209
External Links
PubMed source DOI: 10.1073/pnas.87.6.2157 PMC Full Text View journal details
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