Reactivity of coagulase-negative staphylococci isolated from cow and goat milk with monoclonal antibodies to Staphylococcus aureus capsular polysaccharide types 5 and 8.

Journal of clinical microbiology ·Vol. 28 ·No. 2 ·1990-02-00 ·Pages 358-60

Poutrel B, Mendolia C, Sutra L, Fournier JM

Abstract

Monoclonal antibodies to Staphylococcus aureus capsular polysaccharide types 5 and 8 were used in an enzyme-linked immunosorbent assay to serotype 74 and 42 coagulase-negative isolates from cow and goat milk, respectively. Eighteen (15.5%) isolates were typable: 13 Staphylococcus haemolyticus, 1 S. hyicus, 1 S. simulans, and 1 S. warneri from bovine origin and 2 S. lentus from caprine origin. Type 5 was predominant, accounting for about 89% of typable isolates. Reactivity with monoclonal antibodies varied considerably according to isolates. The significance and the potential importance of these findings are discussed.

MeSH Terms

Animals Antibodies, Bacterial Antibodies, Monoclonal Bacterial Capsules Cattle Coagulase/metabolism Goats Milk/microbiology Polysaccharides, Bacterial/classification,immunology Serotyping Staphylococcus aureus/classification,immunology,isolation & purification

Chemicals

Antibodies, Bacterial Antibodies, Monoclonal Coagulase Polysaccharides, Bacterial capsular polysaccharide, type 5, Staphylococcus aureus

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Poutrel B

Station de Pathologie de la Reproduction, Institut National de la Recherche Agronomique, Nouzilly, France.

Mendolia C

Sutra L

Fournier J M

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21 references, click to expand

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Article Info

Journal

Journal of clinical microbiology

Abbr.

J Clin Microbiol

ISSN

0095-1137

DOI

10.1128/jcm.28.2.358-360.1990

Published

1990-02-00

Pages

358-60

Language

English

Region

United States

NLM ID

7505564

PMCID

PMC269607

Subset

External Links

PubMed source DOI: 10.1128/jcm.28.2.358-360.1990 PMC Full Text View journal details

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Reactivity of coagulase-negative staphylococci isolated from cow and goat milk with monoclonal antibodies to Staphylococcus aureus capsular polysaccharide types 5 and 8.

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