Use of protein A-treated sera in unmasking herpes simplex virus type 1 (HSV-1) immunoglobulin A and identifying HSV-1 immunoglobulin G as the predominant neutralizing antibody.

Journal of clinical microbiology ·Vol. 10 ·No. 4 ·1979-10-00 ·Pages 415-8

Ratner JJ, Sanford BA, Smith KO

Abstract

Treatment of human sera with protein A reduced the amounts of immunoglobulin G (IgG), IgA, and IgM detected by radial immunodiffusion. This treatment also decreased the amount of herpes-specific IgG and IgM detected by radioimmunoassay, whereas it increased and even unmasked the amount of herpes-specific IgA detected. Comparison of protein A-treated sera with untreated sera indicated that herpes simplex virus type 1 IgG was responsible for more than 92 to 99% of the serum neutralizing activity.

MeSH Terms

Antibodies, Viral/analysis Humans Immunodiffusion Immunoglobulin A/analysis Immunoglobulin G/analysis Immunoglobulin M/analysis Neutralization Tests Radioimmunoassay/methods Simplexvirus/immunology Staphylococcal Protein A/immunology

Chemicals

Antibodies, Viral Immunoglobulin A Immunoglobulin G Immunoglobulin M Staphylococcal Protein A

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Ratner J J

Sanford B A

Smith K O

References (12)

12 references, click to expand

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Article Info

Journal

Journal of clinical microbiology

Abbr.

J Clin Microbiol

ISSN

0095-1137

DOI

10.1128/jcm.10.4.415-418.1979

Published

1979-10-00

Pages

415-8

Language

English

Region

United States

NLM ID

7505564

PMCID

PMC273189

Subset

External Links

PubMed source DOI: 10.1128/jcm.10.4.415-418.1979 PMC Full Text View journal details

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Use of protein A-treated sera in unmasking herpes simplex virus type 1 (HSV-1) immunoglobulin A and identifying HSV-1 immunoglobulin G as the predominant neutralizing antibody.

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