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PMID: 23064646 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Structural insights into ligand binding and gene expression control by an adenosylcobalamin riboswitch.

Nature structural & molecular biology ·Vol. 19 ·No. 11 ·2012-11-00 ·Pages 1182-4

Peselis A, Serganov A

Abstract

Coenzyme B(12) has a key role in various enzymatic reactions and controls expression of bacterial genes through riboswitches. Here we report the crystal structure of the Symbiobacterium thermophilum B(12) riboswitch bound to its ligand adenosylcobalamin. The riboswitch forms a unique junctional structure with a large ligand-binding pocket tailored for specific recognition of the adenosyl moiety and flanked by structural elements that stabilize the regulatory region and enable control of gene expression.

MeSH Terms
Base Pairing Chromatography, Ion Exchange Cobamides/chemistry,metabolism Crystallization Electrophoresis, Polyacrylamide Gel Gene Expression Regulation, Bacterial/genetics,physiology Lactobacillales/enzymology Ligands Models, Molecular Protein Conformation Riboswitch/genetics,physiology
Chemicals
Cobamides Ligands Riboswitch cobamamide
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Peselis Alla
Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, New York, New York, USA.
Serganov Alexander
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Article Info
Journal
Nature structural & molecular biology
Abbr.
Nat Struct Mol Biol
ISSN
1545-9985
DOI
10.1038/nsmb.2405
Published
2012-11-00
Epub
2012-00-14
Pages
1182-4
Language
English
Region
United States
NLM ID
101186374
Subset
IM
Databases
PDB
4GXY
Corrections
CommentIn
PMID: 23142981
External Links
PubMed source DOI: 10.1038/nsmb.2405 View journal details
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