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PMID: 2304463 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Expression of a foreign gene in a line of transgenic mice is modulated by a chromosomal position effect.

Molecular and cellular biology ·Vol. 10 ·No. 3 ·1990-03-00 ·Pages 1192-8

al-Shawi R, Kinnaird J, Burke J, Bishop JO

Abstract

Unusual aberrant expression of a foreign gene in a particular transgenic mouse line is often attributed to chromosomal position effect, although proof of this is lacking. An alternative explanation is that expression has been modified by the arrangement of multiple copies of the foreign gene at the insertion site or by mutation or gene rearrangement. We have distinguished between these explanations in the case of one particular transgenic line by recovering the aberrantly expressed foreign DNA and reintroducing it into the mouse genome to produce secondary transgenic mice. The expression pattern of the gene in the secondary transgenic mice was normal, showing that this case of aberrant expression is due to a chromosomal position effect.

MeSH Terms
Animals Blotting, Southern Chromosomes/ultrastructure Cloning, Molecular Gene Expression Regulation, Enzymologic Infertility, Male/genetics Male Mice Mice, Transgenic/genetics Restriction Mapping Thymidine Kinase/genetics Tissue Distribution
Chemicals
Thymidine Kinase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
al-Shawi R
Department of Genetics, University of Edinburgh, Scotland.
Kinnaird J
Burke J
Bishop J O
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39 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.3.1192-1198.1990
Published
1990-03-00
Pages
1192-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360995
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.10.3.1192-1198.1990 PMC Full Text View journal details
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