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题目:: Chikungunya virus induces a more moderate cytopathic effect in mosquito cells than in mammalian cells.
作者:: Li(Yong-Gang),Siripanyaphinyo(Uamporn),Tumkosit(Uranan),Noranate(Nitchakarn),A-nuegoonpipat(Atchareeya),Tao(Ran),Kurosu(Takeshi),Ikuta(Kazuyoshi),Takeda(Naokazu),Anantapreecha(Surapee)
状态:: 发布时间2012-11-27 , 更新时间 2014-11-20
期刊:: Intervirology
摘要:: Chikungunya virus (CHIKV) is an alphavirus belonging to the Togaviridae family. Alphaviruses cause a chronic non-cytopathic infection in mosquito cells, while they develop a highly cytopathic infection in cells originating from various vertebrates. In this study, we compared the cytopathic effect (CPE) induced by CHIKV in Vero cells and a mosquito cell line, C6/36 cells.,CPE and the virus titers were compared between the CHIKV-infected C6/36 and Vero cells. Apoptosis was measured by TUNEL assay, and the differences between the C6/36 and Vero cells were compared.,CHIKV infection induced strong CPE and apoptosis in the Vero cells, but light CPE in the C6/36 cells. The virus titers produced in the C6/36 cells were much higher than those produced in the Vero cells.,The reason CHIKV induced strong CPE is that this virus triggers strong apoptosis in Vero cells compared with C6/36 cells. CHIKV established a persistent infection in C6/36 cells after being passaged 20 times. CHIKV infection in mosquito cells was distinct from that in Vero cells. The cell and species specificity of CHIKV-induced cell death implies that the cellular and viral regulators involved in apoptosis may play an important role in determining the outcome of CHIKV infection.
语言:: eng
DOI:: 10.1159/000339985

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