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PMID: 22890147 Published · ppublish English Journal Article Review

Genomic sequencing of uncultured microorganisms from single cells.

Nature reviews. Microbiology ·Vol. 10 ·No. 9 ·2012-09-00 ·Pages 631-40

Lasken RS

Abstract

Sequencing DNA from single cells has opened new windows onto the microbial world. It is becoming routine to sequence bacterial species directly from environmental samples or clinical specimens without the need to develop cultivation methods. Recent technical improvements often allow nearly complete genome assembly from these otherwise inaccessible species. New bioinformatics methods are also improving genome assembly from single cells. The use of single-cell sequencing in combination with metagenomic analysis is also emerging as a powerful new strategy to analyse bacterial communities. Here, the technical developments that have enabled single-cell sequencing, as well as some of the most exciting applications of this approach from the past few years, are reviewed.

MeSH Terms
Bacteria/genetics Computational Biology/methods DNA, Bacterial/chemistry,genetics Genomics/methods Metagenome Sequence Analysis, DNA/methods
Chemicals
DNA, Bacterial
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Lasken Roger S
J. Craig Venter Institute, La Jolla, California 92121, USA. rlasken@jcvi.org
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Article Info
Journal
Nature reviews. Microbiology
Abbr.
Nat Rev Microbiol
ISSN
1740-1534
DOI
10.1038/nrmicro2857
Email
rlasken@jcvi.org
Published
2012-09-00
Pages
631-40
Language
English
Region
England
NLM ID
101190261
Subset
IM
External Links
PubMed source DOI: 10.1038/nrmicro2857 View journal details
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