Home LiteratureArticle Details
PMID: 22886557 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Yeasts from phylloplane and their capability to produce indole-3-acetic acid.

World journal of microbiology & biotechnology ·Vol. 28 ·No. 12 ·2012-12-00 ·Pages 3323-35

Limtong S, Koowadjanakul N

Abstract

Yeasts were isolated from the phylloplane of various plant species collected from seven provinces in Thailand. A total of 114 yeast strains and 10 strains of a yeast-like fungus were obtained by enrichment isolation from 91 out of 97 leaf samples (93.8 %). On the basis of the D1/D2 domain of the large subunit rRNA gene sequence similarity, 98 strains were identified to be of 36 yeast species in 18 genera belonging to Ascomycota viz. Candida, Clavispora, Cyberlindnera, Debaryomyces, Hanseniaspora, Hyphopichia, Kazachstania, Kluyveromyces, Kodamaea, Lachancea, Metschnikowia, Meyrozyma, Pichia, Starmerella, Torulaspora and Wickerhamomyces, and to Basidiomycota viz. Sporidiobolus and Trichosporon. Three strains were found to represent two novels Candida species which were previously described as C. sirachaensis and C. sakaeoensis. Ten strains of yeast-like fungus were identified as Aureobasidium pullulans of the phylum Ascomycota. Ascomycetous yeast species accounted altogether for 98.0 % of the 98 strains. The prevalent species was Candida tropicalis with a low frequency of isolation (14.3 %). Diversity of yeasts other than ballistoconidium-forming yeast in phylloplane in a tropical country in Asia has been reported for the first time. All strains obtained were accessed for the capability to produce IAA and result revealed that 39 strains in 20 species, one strain each of an undescribed and a novel species, and two unidentified strains showed the capability of producing IAA when cultivated in yeast extract peptone dextrose broth supplemented with 0.1 % L-tryptophan. All five strains of Candida maltosa produced relatively high concentrations of IAA.

MeSH Terms
Cluster Analysis DNA, Fungal/chemistry,genetics DNA, Ribosomal/chemistry,genetics Genes, rRNA Indoleacetic Acids/metabolism Molecular Sequence Data Phylogeny Plant Leaves/microbiology RNA, Fungal/genetics RNA, Ribosomal/genetics Sequence Analysis, DNA Thailand Yeasts/classification,genetics,isolation & purification,metabolism
Chemicals
DNA, Fungal DNA, Ribosomal Indoleacetic Acids RNA, Fungal RNA, Ribosomal RNA, ribosomal, 26S indoleacetic acid
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Limtong Savitree
Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand. fscistl@ku.ac.th
Koowadjanakul Nampueng
References (25)
25 references, click to expand
  1. Candida aechmeae sp. nov. and Candida vrieseae sp. nov., novel yeast species isolated from the phylloplane of bromeliads in Southern Brazil.
    Int J Syst Evol Microbiol. 2010 Jan;60(Pt 1):244-248 PMID: 19648342
  2. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences.
    J Mol Evol. 1980 Dec;16(2):111-20 PMID: 7463489
  3. Three new species of bipolar budding yeasts of the genus Hanseniaspora and its anamorph Kloeckera isolated in Thailand.
    FEMS Yeast Res. 2009 Dec;9(8):1327-37 PMID: 19788563
  4. The neighbor-joining method: a new method for reconstructing phylogenetic trees.
    Mol Biol Evol. 1987 Jul;4(4):406-25 PMID: 3447015
  5. A critical examination of the specificity of the salkowski reagent for indolic compounds produced by phytopathogenic bacteria.
    Appl Environ Microbiol. 1995 Feb;61(2):793-6 PMID: 16534942
  6. Yeast communities associated with sugarcane in Campos, Rio de Janeiro, Brazil.
    Int Microbiol. 1998 Sep;1(3):205-8 PMID: 10943361
  7. Production of indole-3-acetic acid and related indole derivatives from L-tryptophan by Rubrivivax benzoatilyticus JA2.
    Appl Microbiol Biotechnol. 2011 Feb;89(4):1001-8 PMID: 20972782
  8. Suppression of Rhizoctonia solani diseases of sugar beet by antagonistic and plant growth-promoting yeasts.
    J Appl Microbiol. 2004;96(1):69-75 PMID: 14678160
  9. Yeast communities associated with stingless bees.
    FEMS Yeast Res. 2003 Dec;4(3):271-5 PMID: 14654431
  10. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences.
    Antonie Van Leeuwenhoek. 1998 May;73(4):331-71 PMID: 9850420
  11. Kazachstania siamensis sp. nov., an ascomycetous yeast species from forest soil in Thailand.
    Int J Syst Evol Microbiol. 2007 Feb;57(Pt 2):419-422 PMID: 17267990
  12. Candida stigmatis sp. nov., a new anamorphic yeast species isolated from flowers.
    FEMS Yeast Res. 2010 May;10(3):362-5 PMID: 19788562
  13. [Microbial producers of plant growth stimulators and their practical use: A review].
    Prikl Biokhim Mikrobiol. 2006 Mar-Apr;42(2):133-43 PMID: 16761564
  14. Two new ascomycetous anamorphic yeast species related to Candida friedrichii--Candida jaroonii sp. nov., and Candida songkhlaensis sp. nov.--isolated in Thailand.
    Antonie Van Leeuwenhoek. 2008 Aug;94(2):267-76 PMID: 18425597
  15. Biodegradation and metabolism of unusual carbon compounds by anoxygenic phototrophic bacteria.
    Adv Microb Physiol. 1998;39:339-77 PMID: 9328651
  16. Candida sirachaensis sp. nov. and Candida sakaeoensis sp. nov. two anamorphic yeast species from phylloplane in Thailand.
    Antonie Van Leeuwenhoek. 2012 Aug;102(2):221-9 PMID: 22456793
  17. Candida potacharoeniae sp. nov. and Candida spenceri sp. nov., two novel galactose-containing ascomycetous anamorphic yeast species isolated in Thailand.
    J Gen Appl Microbiol. 2010 Aug;56(4):287-95 PMID: 20953093
  18. [Massive isolation of anamorphous ascomycete yeasts Candida oleophila from plant phyllosphere].
    Mikrobiologiia. 2007 Nov-Dec;76(6):896-901 PMID: 18297883
  19. Phylloplane yeasts from Portugal: seven novel anamorphic species in the Tremellales lineage of the Hymenomycetes (Basidiomycota) producing orange-coloured colonies.
    FEMS Yeast Res. 2005 Dec;5(12):1167-83 PMID: 16081324
  20. Wickerhamomyces edaphicus sp. nov. and Pichia jaroonii sp. nov., two ascomycetous yeast species isolated from forest soil in Thailand.
    FEMS Yeast Res. 2009 May;9(3):504-10 PMID: 19228199
  21. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.
    Nucleic Acids Res. 1997 Sep 1;25(17):3389-402 PMID: 9254694
  22. Characterization of three endophytic, indole-3-acetic acid-producing yeasts occurring in Populus trees.
    Mycol Res. 2009 Sep;113(Pt 9):973-80 PMID: 19539760
  23. CONFIDENCE LIMITS ON PHYLOGENIES: AN APPROACH USING THE BOOTSTRAP.
    Evolution. 1985 Jul;39(4):783-791 PMID: 28561359
  24. The Ecology and Biogeography of Microorganisms on Plant Surfaces.
    Annu Rev Phytopathol. 2000 Sep;38:145-180 PMID: 11701840
  25. Ogataea phyllophila sp. nov., Candida chumphonensis sp. nov. and Candida mattranensis sp. nov., three methylotrophic yeast species from phylloplane in Thailand.
    Antonie Van Leeuwenhoek. 2011 Aug;100(2):207-17 PMID: 21479845
Article Info
Journal
World journal of microbiology & biotechnology
Abbr.
World J Microbiol Biotechnol
ISSN
1573-0972
DOI
10.1007/s11274-012-1144-9
Email
fscistl@ku.ac.th
Published
2012-12-00
Epub
2012-00-11
Pages
3323-35
Language
English
Region
Germany
NLM ID
9012472
Subset
IM
Databases
GENBANK
AB617902, AB617903, AB617904, AB617905, AB617906, AB617907, AB617908, AB617909, AB617910, AB617911, AB617912, AB617913, AB617914, AB617915, AB617916, AB617917, AB617918, AB617919, AB617920, AB617921, AB617922, AB617923, AB617924, AB617925, AB617926, AB617927, AB617928, AB617929, AB617930, AB617931, AB617932, AB617933, AB617934, AB617935, AB617936, AB617937, AB617938, AB617939, AB617940, AB617941, AB617942, AB617943, AB617944, AB617945, AB617946, AB617947, AB617948, AB617949, AB617950, AB617951, AB617952, AB617953, AB617954, AB617955, AB617956, AB617957, AB617958, AB617959, AB617960, AB617961, AB617962, AB617963, AB617964, AB617965, AB617966, AB617967, AB617968, AB617969, AB617970, AB617971, AB617972, AB617973, AB617974, AB617975, AB617976, AB617977, AB617978, AB617979, AB617980, AB617981, AB617982, AB617983, AB617984, AB617985, AB617989, AB617990, AB617991, AB617992, AB617995, AB617996, AB617997, AB617998, AB617999, AB618000, AB618001, AB618002, AB618003, AB618004, AB618005, AB618006, AB618007, AB618008, AB618009, AB618010, AB618011, AB618012, AB618013, AB618014, AB618015, AB618016, AB618017, AB618018, AB618019, AB618020, AB618021, AB618022, AB618023, AB618024, AB618025, AB618026, AB618027, AB618028, AB618029, AB618030
External Links
PubMed source DOI: 10.1007/s11274-012-1144-9 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2