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PMID: 22837398 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Fungal endophyte communities reflect environmental structuring across a Hawaiian landscape.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 109 ·No. 32 ·2012-08-07 ·Pages 13022-7

Zimmerman NB, Vitousek PM

Abstract

We surveyed endophytic fungal communities in leaves of a single tree species (Metrosideros polymorpha) across wide environmental gradients (500-5,500 mm of rain/y; 10-22 °C mean annual temperature) spanning short geographic distances on Mauna Loa Volcano, Hawai'i. Using barcoded amplicon pyrosequencing at 13 sites (10 trees/site; 10 leaves/tree), we found very high levels of diversity within sites (a mean of 551 ± 134 taxonomic units per site). However, among-site diversity contributed even more than did within-site diversity to the overall richness of more than 4,200 taxonomic units observed in M. polymorpha, and this among-site variation in endophyte community composition correlated strongly with temperature and rainfall. These results are consistent with suggestions that foliar endophytic fungi are hyperdiverse. They further suggest that microbial diversity may be even greater than has been assumed and that broad-scale environmental controls such as temperature and rainfall can structure eukaryotic microbial diversity. Appropriately constrained study systems across strong environmental gradients present a useful means to understand the environmental factors that structure the diversity of microbial communities.

MeSH Terms
Base Sequence Biota Cluster Analysis Computational Biology DNA Primers/genetics Endophytes/genetics,physiology Environment Fungi/genetics,physiology Genetic Variation Geography Hawaii Molecular Sequence Data Myrtaceae/microbiology Plant Leaves/microbiology Rain Sequence Analysis, DNA Species Specificity Temperature
Chemicals
DNA Primers
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Zimmerman Naupaka B
Department of Biology, Stanford University, Stanford, CA 94305, USA. naupaka@stanford.edu
Vitousek Peter M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
1091-6490
DOI
10.1073/pnas.1209872109
Email
naupaka@stanford.edu
Published
2012-08-07
Epub
2012-00-25
Pages
13022-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC3420199
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.1209872109 PMC Full Text View journal details
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