The influence of 8-Br 3', 5'-cyclic nucleotide analogs and of inhibitors of 3', 5'-cyclic nucleotide phosphodiesterase, on noradrenaline secretion and neuromuscular transmission in guinea-pig vas deferens.

Naunyn-Schmiedeberg's archives of pharmacology ·Vol. 308 ·No. 2 ·1979-08-00 ·Pages 99-105

Stjärne L, Bartfai T, Alberts P

Abstract

The effects of 3', 5'-cyclic nucleotide phosphodiesterase (PDE) inhibitors and of 8-Br 3', 5'-cyclic nucleotide analogs on nerve-muscle transmission were studied in the guinea-pig vas deferens preincubated with 3H-noradrenaline. 8-Br cyclic AMP and the PDE inhibitors 3-isobutyl-1-methylxanthine (IBMX) and 3-propionyl-4-hydrazinopyrazolopyridine (SQ 20006) enhanced the secretion of 3H-NA evoked by transmural nerve stimulation. 8-Br cyclic GMP was without effect in this respect. The muscle contraction evoked by transmural nerve stimulation, high potassium or by application of exogenous noradrenaline was depressed by IBMX and SQ 2006. The contraction evoked by transmural nerve stimulation was enhanced by 8-Br cyclic AMP and depressed by 8-Br cyclic GMP. These findings suggest differential involvement of 3', 5'-adenosine- and guanosine-cyclic nucleotides in excitation-secretion-coupling in the noradrenergic sympathetic nerves, and in excitation-contraction-coupling in the smooth muscle, of guinea-pig vas deferens.

MeSH Terms

3',5'-Cyclic-AMP Phosphodiesterases/antagonists & inhibitors 3',5'-Cyclic-GMP Phosphodiesterases/antagonists & inhibitors Animals Guinea Pigs In Vitro Techniques Male Muscle Contraction/drug effects Neuromuscular Junction/drug effects Norepinephrine/metabolism Nucleotides, Cyclic/pharmacology Synaptic Transmission/drug effects Vas Deferens/innervation,metabolism

Chemicals

Nucleotides, Cyclic 3',5'-Cyclic-AMP Phosphodiesterases 3',5'-Cyclic-GMP Phosphodiesterases Norepinephrine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Stjärne L

Bartfai T

Alberts P

References (16)

16 references, click to expand

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Article Info

Journal

Naunyn-Schmiedeberg's archives of pharmacology

Abbr.

Naunyn Schmiedebergs Arch Pharmacol

ISSN

0028-1298

DOI

10.1007/BF00499050

Published

1979-08-00

Pages

99-105

Language

English

Region

Germany

NLM ID

0326264

Subset

External Links

PubMed source DOI: 10.1007/BF00499050 View journal details

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The influence of 8-Br 3', 5'-cyclic nucleotide analogs and of inhibitors of 3', 5'-cyclic nucleotide phosphodiesterase, on noradrenaline secretion and neuromuscular transmission in guinea-pig vas deferens.

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