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PMID: 227912 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Subcellular distribution in cerebral cortex of two proteins phosphorylated by a cAMP-dependent protein kinase.

The Journal of cell biology ·Vol. 83 ·No. 2 Pt 1 ·1979-11-00 ·Pages 308-19

Ueda T, Greengard P, Berzins K, Cohen RS, Blomberg F, Grab DJ, Siekevitz P

Abstract

The subcellular distribution of Proteins Ia and Ib, two proteins which serve as specific substrates for protein kinases present in mammalian brain, was studied in the dog cerebral cortex. Proteins Ia and Ib were found to be most highly enriched in synaptic vesicle fractions; they were also present in postsynaptic density and synaptic membrane fractions in significant amounts. Proteins Ia and Ib present in the synaptic vesicle fraction appear to be similar, if not identical, to those present in the postsynaptic density fraction as judged by several criteria: (a) the ability to serve as substrate for cAMP-dependent protein kinase, (b) electrophoretic mobility in the presence of sodium dodecyl sulfate, (c) extractability with NH4Cl or EGTA, and (d) fragmentation to electrophoretically similar peptides by a purified Staphylococcus aureus protease. In addition, the postsynaptic density fraction has been found to contain cAMP-dependent Protein Ia and Protein Ib kinase activity. The subcellular localization of Proteins Ia and Ib suggests a role for these proteins in the physiology of the synapse.

MeSH Terms
Animals Cerebral Cortex/analysis,enzymology Cyclic AMP/pharmacology Dogs Phosphoproteins/analysis Protein Kinases/metabolism Subcellular Fractions/analysis,enzymology Synaptic Membranes/analysis Synaptic Vesicles/analysis
Chemicals
Phosphoproteins Cyclic AMP Protein Kinases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Ueda T
Greengard P
Berzins K
Cohen R S
Blomberg F
Grab D J
Siekevitz P
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24 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.83.2.308
Published
1979-11-00
Pages
308-19
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2111539
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.83.2.308 PMC Full Text View journal details
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