Abstract
The Rho GTPase RhoB has been shown to affect cell migration, but how it does this is not clear. Here we show that cells depleted of RhoB by RNAi are rounded and have defects in Rac-mediated spreading and lamellipodium extension, although they have active membrane ruffling around the periphery. Depletion of the exchange factor GEF-H1 induces a similar phenotype. RhoB-depleted cells migrate faster, but less persistently in a chemotactic gradient, and frequently round up during migration. RhoB-depleted cells have similar numbers of focal adhesions to control cells during spreading and migration, but show more diffuse and patchy contact with the substratum. They have lower levels of surface β1 integrin, and β1 integrin activity is reduced in actin-rich protrusions. We propose that RhoB contributes to directional cell migration by regulating β1 integrin surface levels and activity, thereby stabilizing lamellipodial protrusions.
Keywords
Rac1
Rho guanosine triphosphate
RhoB
cytoskeleton
focal adhesions
integrins
MeSH Terms
Adenocarcinoma/pathology
Breast Neoplasms/pathology
Cell Line, Tumor
Cell Movement/physiology
Cell Shape
Female
Focal Adhesions/physiology
Guanine Nucleotide Exchange Factors/physiology
Guanosine Triphosphate/physiology
Humans
Integrin beta1/metabolism
Male
Microtubules/metabolism
Neoplasm Invasiveness
Neoplasm Proteins/physiology
Prostatic Neoplasms/pathology
Pseudopodia/physiology
RNA, Small Interfering/pharmacology
Recombinant Fusion Proteins/physiology
Rho Guanine Nucleotide Exchange Factors
rac1 GTP-Binding Protein/physiology
rhoB GTP-Binding Protein/physiology
Chemicals
ARHGEF2 protein, human
Guanine Nucleotide Exchange Factors
Integrin beta1
Neoplasm Proteins
RAC1 protein, human
RNA, Small Interfering
Recombinant Fusion Proteins
Rho Guanine Nucleotide Exchange Factors
Guanosine Triphosphate
rac1 GTP-Binding Protein
rhoB GTP-Binding Protein
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Vega Francisco M
Randall Division of Cell and Molecular Biophysics, King's College London, New Hunt's House, Guy's Campus, London SE1 1UL, UK.
Colomba Audrey
Reymond Nicolas
Thomas Mairian
Ridley Anne J
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