Home LiteratureArticle Details

PMID: 2272338 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Activity of carcinogens that bind to the C8 position of guanine residues in an assay specific for the detection of -2 frameshift mutations in a defined hot spot.

Environmental health perspectives ·Vol. 88 ·1990-08-00 ·Pages 83-7

Fuchs RP, Bintz R

Abstract

In this paper we describe a reversion assay specific for the detection of -2 frameshift mutations occurring within short stretches of alternating GC sequences. We have compared a series of chemical carcinogens that all bind covalently to the C8 position of guanine residues for their potency in inducing revertants in this assay. Large variations in potency are found within the list of compounds that were tested. The most potent chemicals tested induce the reversion frequency by a factor of 10(5) over background, whereas others only increase it by two orders of magnitude. These differences are discussed in terms of the conformational changes that the different C8-guanine adducts induce in DNA.

MeSH Terms

Base Sequence Binding Sites Carcinogens/metabolism,pharmacology Guanine/metabolism Molecular Conformation Mutagenicity Tests/methods Mutagens

Chemicals

Carcinogens Mutagens Guanine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Fuchs R P

Carcinogenesis and Molecular and Structural Mutagenesis Group, Center for National Scientific Research, Strasbourg, France.

Bintz R

References (20)

20 references, click to expand

Genetic control of AAF-induced mutagenesis at alternating GC sequences: an additional role for RecA.
Mol Gen Genet. 1989 Jan;215(2):306-11 PMID: 2651884
Synthesis and mutagenic activity of nitro-imidazoarenes. A study on the mechanism of the genotoxicity of heterocyclic arylamines and nitroarenes.
Mutagenesis. 1988 Mar;3(2):147-52 PMID: 3132587
Strong structural effect of the position of a single acetylaminofluorene adduct within a mutation hot spot.
Nucleic Acids Res. 1989 Dec 11;17(23):9531-41 PMID: 2602135
Sensitivity of the conformation of deoxyguanosine to binding at the C-8 position by N-acetylated and unacetylated 2-aminofluorene.
Carcinogenesis. 1980;1(11):955-9 PMID: 11219850
On the interaction of N-2-fluorenylhydroxylamine with nucleic acids in vitro.
Biochem Biophys Res Commun. 1965 Sep 22;20(6):793-9 PMID: 5861697
8-(N-2-fluorenylacetamido)guanosine, an arylamidation reaction product of guanosine and the carcinogen N-acetoxy-N-2-fluorenylacetamide in neutral solution.
Biochemistry. 1967 Jan;6(1):177-82 PMID: 6030315
Comparative studies on the 7-iodo and 7-fluoro derivatives of N-acetoxy-N-2-acetylaminofluorene: binding sites on DNA and conformational change of modified deoxytrinucleotides.
Biochemistry. 1978 Jun 27;17(13):2561-7 PMID: 209820
Conformation of poly(dG-dC) . poly(dG-dC) modified by the carcinogens N-acetoxy-N-acetyl-2-aminofluorene and N-hydroxy-N-2-aminofluorene.
Proc Natl Acad Sci U S A. 1980 Aug;77(8):4597-601 PMID: 6933507
Induction of the Z conformation in poly(dG-dC).poly(dG-dC) by binding of N-2-acetylaminofluorene to guanine residues.
Proc Natl Acad Sci U S A. 1981 Mar;78(3):1451-5 PMID: 6262801
Hot spots of frameshift mutations induced by the ultimate carcinogen N-acetoxy-N-2-acetylaminofluorene.
Nature. 1981 Dec 17;294(5842):657-9 PMID: 7031481
Proteins required for ultraviolet light and chemical mutagenesis. Identification of the products of the umuC locus of Escherichia coli.
J Mol Biol. 1983 Feb 25;164(2):175-92 PMID: 6302271
Carcinogen-induced mutation spectrum in wild-type, uvrA and umuC strains of Escherichia coli. Strain specificity and mutation-prone sequences.
J Mol Biol. 1984 Jul 25;177(1):33-51 PMID: 6379196
Conformations of poly(dG-dC).poly(dG-dC) modified by the O-acetyl derivative of the carcinogen 4-hydroxyaminoquinoline 1-oxide.
Nucleic Acids Res. 1984 Oct 25;12(20):7915-27 PMID: 6093058
Conformational changes induced in DNA by the in vitro reaction with the mutagenic amine: 3-N,N-acetoxyacetylamino-4,6-dimethyldipyrido (1,2-a: 3', 2'-d) imidazole.
Nucleic Acids Res. 1984 Nov 26;12(22):8553-66 PMID: 6548807
Adducts from in vivo action of the carcinogen 4-hydroxyaminoquinoline 1-oxide in rats and from in vitro reaction of 4-acetoxyaminoquinoline 1-oxide with DNA and polynucleotides.
Cancer Res. 1985 Feb;45(2):520-5 PMID: 3917848
Reaction of DNA with a mutagenic 3-N,N-acetoxyacetylamino-4,6-dimethyldipyrido[1,2-a:3',2'- d]imidazole (N-AcO-AGlu-P-3) related to glutamic acid pyrolysates.
Carcinogenesis. 1985 Mar;6(3):377-83 PMID: 2983901
DNA binding and mutation spectra of the carcinogen N-2-aminofluorene in Escherichia coli. A correlation between the conformation of the premutagenic lesion and the mutation specificity.
J Mol Biol. 1985 Jun 5;183(3):341-51 PMID: 3894674
Construction of frameshift mutation hot spots within the tetracycline resistance gene of pBR322.
Biochimie. 1985 Mar-Apr;67(3-4):385-9 PMID: 2994756
Mutation spectrum of the mutagen 3-N,N-acetoxyacetylamino-4,6-dimethyldipyrido[1,2-a:3',2'- d]imidazole in Escherichia coli.
Carcinogenesis. 1988 Jan;9(1):183-5 PMID: 3275503
Single adduct mutagenesis: strong effect of the position of a single acetylaminofluorene adduct within a mutation hot spot.
Proc Natl Acad Sci U S A. 1989 Jun;86(11):4147-51 PMID: 2657743

Article Info

Journal

Environmental health perspectives

Abbr.

Environ Health Perspect

ISSN

0091-6765

DOI

10.1289/ehp.908883

Published

1990-08-00

Pages

83-7

Language

English

Region

United States

NLM ID

0330411

PMCID

PMC1567979

Subset

External Links

PubMed source DOI: 10.1289/ehp.908883 PMC Full Text View journal details

Analysis Services

Activity of carcinogens that bind to the C8 position of guanine residues in an assay specific for the detection of -2 frameshift mutations in a defined hot spot.

Contact

WeChat Official Account