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PMID: 22708488 Published · ppublish English Journal Article Research Support, N.I.H., Intramural Review

Functional interplay between RNA-binding protein HuR and microRNAs.

Current protein & peptide science ·Vol. 13 ·No. 4 ·2012-06-00 ·Pages 372-9

Srikantan S, Tominaga K, Gorospe M

Abstract

The mammalian RNA-binding protein (RBP) HuR associates with numerous mRNAs encoding proteins with roles in cell division, cell survival, immune response, and differentiation. HuR was known to stabilize many of these mRNAs and/or modulated their translation, but the molecular processes by which HuR affected the fate of target mRNAs was largely unknown. Evidence accumulated over the past five years has revealed that the influence of HuR on many bound transcripts depends on HuR's interplay with microRNAs which associate with the same mRNAs. Here, we review the interactions of HuR and microRNAs - both competitive and cooperative - that govern expression of shared target mRNAs. Competition between HuR and microRNAs typically results in enhanced gene expression if the HuR-mRNA interaction prevails, and in repression if the microRNA remains associated. Cooperation between HuR and microRNAs leads to lower expression of the shared mRNA. We also describe the regulation of HuR levels by microRNAs as well as the regulation of microRNA levels by HuR. Finally, we discuss transcriptome-wide analyses of HuR-bound mRNAs with neighboring microRNA sites, and review the emerging mechanisms whereby microRNAs confer versatility and robustness to the post-transcriptional outcomes of HuR targets.

MeSH Terms
ELAV Proteins/genetics,metabolism Gene Expression Regulation Humans MicroRNAs/genetics,metabolism Protein Binding/genetics RNA, Messenger/genetics,metabolism
Chemicals
ELAV Proteins MicroRNAs RNA, Messenger
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Srikantan Subramanya
Laboratory of Molecular Biology and Immunology, National Institute on Aging-Intramural Research Program, NIH, Baltimore, MD 21224, USA.
Tominaga Kumiko
Gorospe Myriam
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Article Info
Journal
Current protein & peptide science
Abbr.
Curr Protein Pept Sci
ISSN
1875-5550
DOI
10.2174/138920312801619394
Published
2012-06-00
Pages
372-9
Language
English
Region
United Arab Emirates
NLM ID
100960529
PMCID
PMC3535178
Subset
IM
Grants
Intramural NIH HHS · ZIA AG000511-14 · United States
External Links
PubMed source DOI: 10.2174/138920312801619394 PMC Full Text View journal details
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