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PMID: 227059 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A sign inversion mechanism for enzymatic supercoiling of DNA.

Science (New York, N.Y.) ·Vol. 206 ·No. 4422 ·1979-11-30 ·Pages 1081-3

Brown PO, Cozzarelli NR

Abstract

Both the introduction and the removal of supertwists by DNA gyrase change the linking number of DNA in steps of two. This surprising finding provides strong evidence that gyrase acts by a mechanism, called sign inversion, whereby a positive supercoil is directly inverted into a negative one via a transient double-strand break.

MeSH Terms
Adenosine Triphosphate/metabolism DNA Topoisomerases, Type II/metabolism DNA, Superhelical Escherichia coli/enzymology Models, Biological Nucleic Acid Conformation
Chemicals
DNA, Superhelical Adenosine Triphosphate DNA Topoisomerases, Type II
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Brown P O
Cozzarelli N R
Article Info
Journal
Science (New York, N.Y.)
Abbr.
Science
ISSN
0036-8075
DOI
10.1126/science.227059
Published
1979-11-30
Pages
1081-3
Language
English
Region
United States
NLM ID
0404511
Subset
IM
External Links
PubMed source DOI: 10.1126/science.227059 View journal details
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