Detection of the Philadelphia chromosome in interphase nuclei.

Cytogenetics and cell genetics ·Vol. 54 ·No. 3-4 ·1990-00-00 ·Pages 108-11

Arnoldus EP, Wiegant J, Noordermeer IA, Wessels JW, Beverstock GC, Grosveld GC, van der Ploeg M, Raap AK

Abstract

Double fluorescence in situ hybridization was used to detect Philadelphia (Ph) chromosomes in interphase nuclei and metaphases of patients with chronic myeloid leukemia. Application of cosmid probes for 3' ABL and 5' BCR sequences gave better results than libraries for chromosomes 9 and 22. The present approach may provide an alternative method for monitoring minimal residual disease in Ph+ CML patients.

MeSH Terms

Cosmids Humans Immunohistochemistry Interphase Karyotyping Leukemia, Myelogenous, Chronic, BCR-ABL Positive/genetics Philadelphia Chromosome

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Arnoldus E P

Department of Cytochemistry and Cytometry, Leiden University, The Netherlands.

Wiegant J

Noordermeer I A

Wessels J W

Beverstock G C

Grosveld G C

van der Ploeg M

Raap A K

Article Info

Journal

Cytogenetics and cell genetics

Abbr.

Cytogenet Cell Genet

ISSN

0301-0171

DOI

10.1159/000132972

Published

1990-00-00

Pages

108-11

Language

English

Region

Switzerland

NLM ID

0367735

Subset

External Links

PubMed source DOI: 10.1159/000132972

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Detection of the Philadelphia chromosome in interphase nuclei.

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