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PMID: 22619176 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Central role of mitofusin 2 in autophagosome-lysosome fusion in cardiomyocytes.

The Journal of biological chemistry ·Vol. 287 ·No. 28 ·2012-07-06 ·Pages 23615-25

Zhao T, Huang X, Han L, Wang X, Cheng H, Zhao Y, Chen Q, Chen J, Cheng H, Xiao R, Zheng M

Abstract

In the heart, autophagy has been implicated in cardioprotection and ischemia-reperfusion tolerance, and the dysregulation of autophagy is associated with the development of heart failure. Mitochondrial dynamic proteins are profoundly involved in autophagic processes, especially the initiation and formation of autophagosomes, but it is not clear whether they play any role in cardiac autophagy. We previously reported that mitofusin 2 (MFN2), a mitochondrial outer membrane protein, serves as a major determinant of cardiomyocyte apoptosis mediated by oxidative stress. Here, we reveal a novel and essential role of MFN2 in mediating cardiac autophagy. We found that specific deletion of MFN2 in cardiomyocytes caused extensive accumulation of autophagosomes. In particular, the fusion of autophagosomes with lysosomes, a critical step in autophagic degradation, was markedly retarded without altering the formation of autophagosomes and lysosomes in response to ischemia-reperfusion stress. Importantly, MFN2 co-immunoprecipitated with RAB7 in the heart, and starvation further increased it. Knockdown of MFN2 by shRNA prevented, whereas re-expression of MFN2 restored, the autophagosome-lysosome fusion in neonatal cardiomyocytes. Hearts from cardiac-specific MFN2 knock-out mice had abnormal mitochondrial and cellular metabolism and were vulnerable to ischemia-reperfusion challenge. Our study defined a novel and essential role of MFN2 in the cardiac autophagic process by mediating the maturation of autophagy at the phase of autophagosome-lysosome fusion; deficiency of MFN2 caused multiple molecular and functional defects that undermined cardiac reserve and gradually led to cardiac vulnerability and dysfunction.

MeSH Terms
Animals Animals, Newborn Autophagy/physiology Blotting, Western Cells, Cultured DNA, Mitochondrial/genetics Echocardiography GTP Phosphohydrolases/deficiency,genetics,metabolism Heart/physiopathology Immunoprecipitation Lysosomes/metabolism Membrane Fusion/physiology Mice Mice, Knockout Microscopy, Electron, Transmission Mitochondria, Heart/genetics,metabolism,ultrastructure Myocardium/metabolism,pathology,ultrastructure Myocytes, Cardiac/enzymology,physiology Phagosomes/metabolism Protein Binding RNA Interference Reperfusion Injury/physiopathology rab GTP-Binding Proteins/metabolism rab7 GTP-Binding Proteins
Chemicals
DNA, Mitochondrial rab7 GTP-Binding Proteins rab7 GTP-binding proteins, mouse GTP Phosphohydrolases Mfn2 protein, mouse rab GTP-Binding Proteins
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Zhao Ting
Institute of Molecular Medicine, State Key Laboratory of Biomembrane and Membrane Biotechnology, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China.
Huang Xiaohu
Han Liang
Wang Xianhua
Cheng Hongqiang
Zhao Yungang
Chen Quan
Chen Ju
Cheng Heping
Xiao Ruiping
Zheng Ming
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Article Info
Journal
The Journal of biological chemistry
Abbr.
J Biol Chem
ISSN
1083-351X
Published
2012-07-06
Epub
2012-00-22
Pages
23615-25
Language
English
Region
United States
NLM ID
2985121R
PMCID
PMC3390636
Subset
IM
Grants
NHLBI NIH HHS · R01 HL066100 · United States
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