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PMID: 22610688 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Review

In vivo protein complex topologies: sights through a cross-linking lens.

Proteomics ·Vol. 12 ·No. 10 ·2012-05-00 ·Pages 1565-75

Bruce JE

Abstract

Proteins are a remarkable class of molecules that exhibit wide diversity of shapes or topological features that underpin protein interactions and give rise to biological function. In addition to quantitation of abundance levels of proteins in biological systems under a variety of conditions, the field of proteome research has as a primary mission the assignment of function for proteins and if possible, illumination of factors that enable function. For many years, chemical cross-linking methods have been used to provide structural data on single purified proteins and purified protein complexes. However, these methods also offer the alluring possibility to extend capabilities to complex biological samples such as cell lysates or intact living cells where proteins may exhibit native topological features that do not exist in purified form. Recent efforts are beginning to provide glimpses of protein complexes and topologies in cells that suggest continued development will yield novel capabilities to view functional topological features of many proteins and complexes as they exist in cells, tissues, or other complex samples. This review will describe rationale, challenges, and a few success stories along the path of development of cross-linking technologies for measurement of in vivo protein interaction topologies.

MeSH Terms
Cross-Linking Reagents/chemistry Protein Conformation Protein Interaction Mapping/methods Proteins/analysis,chemistry,metabolism Proteomics/methods
Chemicals
Cross-Linking Reagents Proteins
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Bruce James E
Department of Genome Sciences, University of Washington, Seattle, WA 98109, USA. jimbruce@u.washington.edu
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Article Info
Journal
Proteomics
Abbr.
Proteomics
ISSN
1615-9861
DOI
10.1002/pmic.201100516
Email
jimbruce@u.washington.edu
Published
2012-05-00
Pages
1565-75
Language
English
Region
Germany
NLM ID
101092707
PMCID
PMC3654877
Subset
IM
Grants
NIGMS NIH HHS · R01 GM097112 · United States
NIGMS NIH HHS · 1R01GM097112 · United States
NHLBI NIH HHS · R01 HL110879 · United States
NIGMS NIH HHS · R01 GM086688 · United States
NHLBI NIH HHS · 1R01HL110879 · United States
NCRR NIH HHS · R01 RR023334 · United States
NIGMS NIH HHS · 5R01GM086688 · United States
NCRR NIH HHS · 5R01RR023334 · United States
External Links
PubMed source DOI: 10.1002/pmic.201100516 PMC Full Text View journal details
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