Are polyphosphoinositides associated with glycophorin in human erythrocyte membranes?

The Biochemical journal ·Vol. 179 ·No. 2 ·1979-05-01 ·Pages 441-4

Shukla SD, Coleman R, Finean JB, Michell RH

Abstract

Glycophorin prepared by a lithium di-iodosalicylate-extraction/phenol-partition method was rich in polyphosphoinositides (phosphatidyl-myo-inositol 4-phosphate and phosphatidyl-myo-inositol 4,5-bisphosphate), but glycophorin extracted by Triton X-100 showed no such enrichment. The enrichment observed in the former preparations appeared not to be caused by pre-existing association between glycophorin and polyphosphoinositides in the human erythrocyte membrane, but to be largely a consequence of the preparative procedures.

MeSH Terms

Glycophorins/isolation & purification Humans Phosphatidylinositols/blood Phospholipids/blood Sialoglycoproteins/isolation & purification

Chemicals

Glycophorins Phosphatidylinositols Phospholipids Sialoglycoproteins

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Shukla S D

Coleman R

Finean J B

Michell R H

References (13)

13 references, click to expand

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Structural analysis of a membrane glycoprotein: glycophorin A.
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31P nuclear magnetic resonance evidence for polyphosphoinositide associated with the hydrophobic segment of glycophorin A.
Biochemistry. 1977 Apr 5;16(7):1317-20 PMID: 191066
The use of phospholipase c to detect structural changes in the membranes of human erythrocytes aged by storage.
Biochim Biophys Acta. 1978 Sep 22;512(2):341-9 PMID: 213113
A calcium-activated polyphosphoinositide phosphodiesterase in the plasma membrane of human and rabbit erythrocytes.
Biochim Biophys Acta. 1978 Apr 4;508(2):277-86 PMID: 205246
Coisolation of glycophorin A and polyphosphoinositides from human erythrocyte membranes.
Can J Biochem. 1978 May;56(5):349-51 PMID: 208725
Phosphorylation in membranes of intact human erythrocytes.
J Biol Chem. 1977 Jan 25;252(2):508-17 PMID: 188816
Freeze-fracture appearance and disposition of band 3 protein from the human erythrocyte membrane in lipid vesicles.
Eur J Biochem. 1978 Apr;85(1):255-61 PMID: 639819
Evidence for the preferential interaction of glycophorin with negatively charged phospholipids.
Biochim Biophys Acta. 1977 Feb 4;464(3):482-92 PMID: 836822
Myo-inositol lipids.
Vitam Horm. 1975;33:529-73 PMID: 180679
The visualization of human erythrocyte membrane proteins and glycoproteins in SDS polyacrylamide gels employing a single staining procedure.
Anal Biochem. 1976 Mar;71(1):223-30 PMID: 1275228
Glycoproteins: isolation from cellmembranes with lithium diiodosalicylate.
Science. 1971 Dec 17;174(4015):1247-8 PMID: 5133448
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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1790441

Published

1979-05-01

Pages

441-4

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1186642

Subset

External Links

PubMed source DOI: 10.1042/bj1790441 PMC Full Text View journal details

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Are polyphosphoinositides associated with glycophorin in human erythrocyte membranes?

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