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PMID: 22579254 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, N.I.H., Intramural Research Support, Non-U.S. Gov't

Solution structure of the ESCRT-I and -II supercomplex: implications for membrane budding and scission.

Structure (London, England : 1993) ·Vol. 20 ·No. 5 ·2012-05-09 ·Pages 874-86

Boura E, Różycki B, Chung HS, Herrick DZ, Canagarajah B, Cafiso DS, Eaton WA, Hummer G, Hurley JH

Abstract

The ESCRT-I and ESCRT-II supercomplex induces membrane buds that invaginate into the lumen of endosomes, a process central to the lysosomal degradation of ubiquitinated membrane proteins. The solution conformation of the membrane-budding ESCRT-I-II supercomplex from yeast was refined against small-angle X-ray scattering (SAXS), single-molecule Förster resonance energy transfer (smFRET), and double electron-electron resonance (DEER) spectra. These refinements yielded an ensemble of 18 ESCRT-I-II supercomplex structures that range from compact to highly extended. The crescent shapes of the ESCRT-I-II supercomplex structures provide the basis for a detailed mechanistic model, in which ESCRT-I-II stabilizes membrane buds and coordinates cargo sorting by lining the pore of the nascent bud necks. The hybrid refinement used here is general and should be applicable to other dynamic multiprotein assmeblies.

MeSH Terms
Cell Membrane/metabolism Crystallography, X-Ray Endosomal Sorting Complexes Required for Transport/chemistry,metabolism Endosomes/metabolism Fluorescence Resonance Energy Transfer Models, Molecular Protein Structure, Secondary Saccharomyces cerevisiae/metabolism Scattering, Small Angle Solutions
Chemicals
Endosomal Sorting Complexes Required for Transport Solutions
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Boura Evzen
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
Różycki Bartosz
Chung Hoi Sung
Herrick Dawn Z
Canagarajah Bertram
Cafiso David S
Eaton William A
Hummer Gerhard
Hurley James H
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Article Info
Journal
Structure (London, England : 1993)
Abbr.
Structure
ISSN
1878-4186
DOI
10.1016/j.str.2012.03.008
Published
2012-05-09
Pages
874-86
Language
English
Region
United States
NLM ID
101087697
PMCID
PMC3572543
Subset
IM
Grants
NIGMS NIH HHS · P01 GM072694 · United States
Intramural NIH HHS · ZIA DK036123-06 · United States
NIGMS NIH HHS · GM072694 · United States
External Links
PubMed source DOI: 10.1016/j.str.2012.03.008 PMC Full Text View journal details
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