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PMID: 2256782 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Catabolism of 3-hydroxybenzoate by the gentisate pathway in Klebsiella pneumoniae M5a1.

Archives of microbiology ·Vol. 154 ·No. 5 ·1990-00-00 ·Pages 489-95

Jones DC, Cooper RA

Abstract

Growth of Klebsiella pneumoniae M5a1 on 3-hydroxybenzoate leads to the induction of 3-hydroxybenzoate monooxygenase, 2,5-dihydroxybenzoate dioxygenase, maleylpyruvate isomerase and fumaryl-pyruvate hydrolase. Growth in the presence of 2,5-dihydroxybenzoate also induces all of these enzymes including the 3-hydroxybenzoate monooxygenase which is not required for 2,5-dihydroxybenzoate catabolism. Mutants defective in 3-hydroxybenzoate monooxygenase fail to grow on 3-hydroxybenzoate but grow normally on 2,5-dihydroxybenzoate. Mutants lacking maleylpyruvate isomerase fail to grow on 3-hydroxybenzoate and 2,5-dihydroxybenzoate. Both kinds of mutants grow normally on 3,4-dihydroxybenzoate. Mutants defective in maleylpyruvate isomerase accumulate maleylpyruvate when exposed to 3-hydroxybenzoate and growth is inhibited. Secondary mutants that have additionally lost 3-hydroxybenzoate monooxygenase are no longer inhibited by the presence of 3-hydroxybenzoate. The 3-hydroxybenzoate monooxygenase gene (mhbM) and the maleylpyruvate isomerase gene (mhbI) are 100% co-transducible by P1 phage.

Related Genes
mhbI mhbM
MeSH Terms
Chromatography, Ion Exchange Gentisates/metabolism Hydroxybenzoates/metabolism Isomerases/genetics,metabolism Klebsiella pneumoniae/enzymology,genetics,growth & development,metabolism Mixed Function Oxygenases/genetics,metabolism Mutation Oxidation-Reduction Transduction, Genetic cis-trans-Isomerases
Chemicals
Gentisates Hydroxybenzoates 3-hydroxybenzoic acid protocatechuic acid Mixed Function Oxygenases 3-hydroxybenzoate-4-monooxygenase Isomerases cis-trans-Isomerases maleylpyruvate isomerase 2,5-dihydroxybenzoic acid
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Jones D C
Department of Biochemistry, University of Leicester, UK.
Cooper R A
References (22)
22 references, click to expand
  1. The formation of phenol in the degradation of p-hydroxybenzoic acid by Klebsiella aerogenes (Aerobacter aerogenes).
    Antonie Van Leeuwenhoek. 1969;35(1):53-64 PMID: 5305796
  2. 2-Phenylethylamine catabolism by Escherichia coli K12.
    J Gen Microbiol. 1987 Feb;133(2):347-51 PMID: 3309152
  3. Metabolic function and properties of 4-hydroxyphenylacetic acid 1-hydroxylase from Pseudomonas acidovorans.
    J Bacteriol. 1975 Jan;121(1):272-85 PMID: 234937
  4. Protocatechuate is not metabolized via catechol in Enterobacter aerogenes.
    J Bacteriol. 1987 Dec;169(12):5827-30 PMID: 3680179
  5. Molecular cloning, expression, and analysis of the genes of the homoprotocatechuate catabolic pathway of Escherichia coli C.
    J Bacteriol. 1988 Nov;170(11):5317-24 PMID: 3053656
  6. Bacterial degradation of 4-hydroxyphenylacetic acid and homoprotocatechuic acid.
    J Bacteriol. 1974 Oct;120(1):159-67 PMID: 4420192
  7. The genetic complexity of nitrogen fixation. The ninth Fleming lecture.
    J Gen Microbiol. 1984 Nov;130(11):2745-55 PMID: 6396374
  8. Purification and some properties of maleylpyruvate hydrolase and fumarylpyruvate hydrolase from Pseudomonas alcaligenes.
    J Bacteriol. 1980 Jul;143(1):70-7 PMID: 7400101
  9. Determination of serum proteins by means of the biuret reaction.
    J Biol Chem. 1949 Feb;177(2):751-66 PMID: 18110453
  10. 3-Hydroxybenzoate 6-hydroxylase from Pseudomonas aeruginosa.
    Biochem Biophys Res Commun. 1973 Dec 10;55(3):897-903 PMID: 4357436
  11. The non-oxidative decarboxylation of p-hydroxybenzoic acid, gentisic acid, protocatechuic acid and gallic acid by Klebsiella aerogenes (Aerobacter aerogenes).
    Antonie Van Leeuwenhoek. 1969;35(3):325-43 PMID: 5309907
  12. The enzymic oxidation of gentisic acid.
    Biochim Biophys Acta. 1959 Jul;34:117-23 PMID: 14413308
  13. A biochemical approach to some problems of environmental pollution.
    Essays Biochem. 1975;11:81-138 PMID: 765127
  14. Catabolism of 3- and 4-hydroxyphenylacetate by the 3,4-dihydroxyphenylacetate pathway in Escherichia coli.
    J Bacteriol. 1980 Jul;143(1):302-6 PMID: 6995433
  15. Catabolism of phenylpropionic acid and its 3-hydroxy derivative by Escherichia coli.
    J Bacteriol. 1983 Jul;155(1):113-21 PMID: 6345502
  16. The metabolism of beta-phenylpropionic acid by an Achromobacter.
    Biochem J. 1965 Dec;97(3):643-50 PMID: 5881653
  17. The metabolism of aromatic acids by Pseudomonas testosteroni and P. acidovorans.
    Arch Mikrobiol. 1967;59(1):302-14 PMID: 5602468
  18. Isolation and mapping of Escherichia coli K12 mutants defective in phenylacetate degradation.
    J Gen Microbiol. 1985 Oct;131(10):2753-7 PMID: 3906029
  19. Bacteriology and nutrition of environmental strains of Klebsiella pneumoniae involved in wood and bark decay.
    Ann Microbiol (Paris). 1983 Mar-Apr;134A(2):189-96 PMID: 6346982
  20. Transduction of the nitrogen-fixation genes in Klebsiella pneumoniae.
    Proc Natl Acad Sci U S A. 1971 Jun;68(6):1174-7 PMID: 5288365
  21. Enzymic cis-trans isomerization of maleylpyruvic acid.
    J Biol Chem. 1961 Nov;236:2835-40 PMID: 14461395
  22. An Escherichia coli mutant defective in the NAD-dependent succinate semialdehyde dehydrogenase.
    Arch Microbiol. 1982 Sep;132(3):270-5 PMID: 6756331
Article Info
Journal
Archives of microbiology
Abbr.
Arch Microbiol
ISSN
0302-8933
DOI
10.1007/BF00245233
Published
1990-00-00
Pages
489-95
Language
English
Region
Germany
NLM ID
0410427
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00245233 View journal details
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