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PMID: 2253904 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Relation of Helicobacter pylori to the human gastric mucosa in chronic gastritis of the antrum.

Gut ·Vol. 31 ·No. 11 ·1990-11-00 ·Pages 1230-6

Thomsen LL, Gavin JB, Tasman-Jones C

Abstract

The spatial relations between bacteria and the affected tissues can indicate pathogenic mechanisms. This study was undertaken to define the spatial relation of Helicobacter pylori to the human gastric mucosa. Antibodies against gastric mucus and ruthenium red were used to stabilise the glycoprotein structure of the mucus and glycocalyces in antral biopsy specimens from eight patients infected with H pylori. The location of organisms and ultrastructural features were assessed using systematic scanning and transmission electron microscopy: 92 (2)% (mean (SE] of H pylori were in the pit mucus, and 7 (3)% were in the surface mucus; 60 (12)% of H pylori were close to epithelial cells, with only 5 (2)% located near the epithelial intercellular junctions. Fine filamentous strands extended between organisms and nearby epithelial cells, with few organisms in membrane to membrane contact. H pylori were not observed between, beneath, or within cells of the gastric mucosa. The preferred location of H pylori in the gastric antrum is within the pit mucus close to the epithelial cell surface, with no evidence that they have a direct toxic effect on the mucosa.

MeSH Terms

Gastric Mucosa/microbiology,ultrastructure Gastritis/microbiology Helicobacter pylori/pathogenicity,ultrastructure Humans Microscopy, Electron Microscopy, Electron, Scanning Pyloric Antrum/microbiology,ultrastructure

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Thomsen L L

Department of Pathology, University of Auckland School of Medicine, New Zealand.

Gavin J B

Tasman-Jones C

References (25)

25 references, click to expand

Local immune response to gastric Campylobacter in non-ulcer dyspepsia.
J Clin Pathol. 1986 Aug;39(8):863-70 PMID: 3528230
Location of bacteria in the mid-colon of the rat.
Appl Environ Microbiol. 1986 Mar;51(3):604-8 PMID: 2938542
Campylobacter like organisms in duodenal and antral endoscopic biopsies: relationship to inflammation.
Gut. 1986 Oct;27(10):1132-7 PMID: 3781324
Simplified techniques for identifying Campylobacter pyloridis.
J Clin Pathol. 1986 Nov;39(11):1279 PMID: 2432094
Ingestion of Campylobacter pyloridis causes gastritis and raised fasting gastric pH.
Am J Gastroenterol. 1987 Mar;82(3):192-9 PMID: 3826027
Growth advantage and enhanced toxicity of Escherichia coli adherent to tissue culture cells due to restricted diffusion of products secreted by the cells.
J Clin Invest. 1987 Apr;79(4):1210-6 PMID: 3031133
Campylobacter pyloridis gastritis I: Detection of urease as a marker of bacterial colonization and gastritis.
Am J Gastroenterol. 1987 Apr;82(4):292-6 PMID: 2436470
Gastric Campylobacter-like organisms, gastritis, and peptic ulcer disease.
Gastroenterology. 1987 Aug;93(2):371-83 PMID: 3297911
Back-scattered electron imaging of sections through the cochlea: a new technique for studying cochlear morphology.
Stain Technol. 1987 May;62(3):191-9 PMID: 2441496
Distribution of Campylobacter pylori in the human stomach obtained at postmortem.
Scand J Gastroenterol. 1988 Apr;23(3):257-64 PMID: 3387889
Pathogenetic implications of ultrastructural findings in Campylobacter pylori related gastroduodenal disease.
Scand J Gastroenterol Suppl. 1988;142:25-39 PMID: 3166531
Ammonia produced by Campylobacter pylori neutralizes H+ moving through gastric mucus.
Scand J Gastroenterol. 1989 Aug;24(6):761-8 PMID: 2814342
Staining of tissue sections for electron microscopy with heavy metals.
J Biophys Biochem Cytol. 1958 Jul 25;4(4):475-8 PMID: 13563554
The histological diagnosis of chronic gastritis in fibreoptic gastroscope biopsy specimens.
J Clin Pathol. 1972 Jan;25(1):1-11 PMID: 5015372
Morphological stabilization of capsules of group B streptococci, types Ia, Ib, II, and III, with specific antibody.
J Bacteriol. 1979 May;138(2):609-17 PMID: 374399
Bacterial adherence: adhesin-receptor interactions mediating the attachment of bacteria to mucosal surface.
J Infect Dis. 1981 Mar;143(3):325-45 PMID: 7014727
Mechanisms of association of bacteria with mucosal surfaces.
Ciba Found Symp. 1981;80:36-55 PMID: 7021088
The role of bacterial surface structures in pathogenesis.
Crit Rev Microbiol. 1981;8(4):303-38 PMID: 6115736
Evidence for a bladder cell glycolipid receptor for Escherichia coli and the effect of neuraminic acid and colominic acid on adherence.
Infect Immun. 1981 Dec;34(3):944-8 PMID: 6277793
Unidentified curved bacilli on gastric epithelium in active chronic gastritis.
Lancet. 1983 Jun 4;1(8336):1273-5 PMID: 6134060
Spiral organisms in endoscopic biopsies of the human stomach.
J Clin Pathol. 1984 Jan;37(1):23-6 PMID: 6707219
Campylobacter like organisms on the gastric mucosa: culture, histological, and serological studies.
J Clin Pathol. 1984 Sep;37(9):1002-6 PMID: 6470176
Campylobacter pyloridis in peptic ulcer disease: microbiology, pathology, and scanning electron microscopy.
Gut. 1985 Nov;26(11):1183-8 PMID: 4065695
Campylobacter pyloridis and gastritis: association with intercellular spaces and adaptation to an environment of mucus as important factors in colonization of the gastric epithelium.
J Infect Dis. 1986 Apr;153(4):658-63 PMID: 3950447
Seroepidemiology of Campylobacter pyloridis.
N Z Med J. 1986 Sep 10;99(809):657-9 PMID: 3463897

Article Info

Journal

Gut

Abbr.

Gut

ISSN

0017-5749

DOI

10.1136/gut.31.11.1230

Published

1990-11-00

Pages

1230-6

Language

English

Region

England

NLM ID

2985108R

PMCID

PMC1378690

Subset

External Links

PubMed source DOI: 10.1136/gut.31.11.1230 PMC Full Text View journal details

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Relation of Helicobacter pylori to the human gastric mucosa in chronic gastritis of the antrum.

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