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PMID: 2250661 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Integration host factor bends the DNA in the Escherichia coli ilvBN promoter region.

Molecular & general genetics : MGG ·Vol. 223 ·No. 2 ·1990-09-00 ·Pages 349-52

Tsui P, Freundlich M

Abstract

Integration host factor (IHF) of Escherichia coli is a site-specific DNA binding protein involved in a wide variety of physiological activities in E. coli and its phages and plasmids. We have previously found that IHF binds specifically to a site just upstream from the ilvBN promoter and strongly decreases transcriptional pausing and termination in the ilvBN leader. In this work we show by gel retardation analysis that IHF binds to bent ilvBN DNA and greatly enhances the bend located within or near the IHF binding site. These data are consistent with the hypothesis that IHF-induced alterations in the conformation of ilvBN promoter-leader DNA is a key to its antitermination activity in this system.

MeSH Terms
Bacterial Proteins/metabolism Base Sequence DNA, Bacterial/genetics,metabolism DNA-Binding Proteins/metabolism Escherichia coli/genetics Integration Host Factors Molecular Sequence Data Promoter Regions, Genetic Restriction Mapping
Chemicals
Bacterial Proteins DNA, Bacterial DNA-Binding Proteins Integration Host Factors
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Tsui P
Department of Biochemistry, State University of New York, Stony Brook 11794-5215.
Freundlich M
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28 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00265076
Published
1990-09-00
Pages
349-52
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIGMS NIH HHS · GM17152 · United States
External Links
PubMed source DOI: 10.1007/BF00265076
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