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PMID: 2247063 Published · ppublish English Journal Article

The SCL gene is formed from a transcriptionally complex locus.

Molecular and cellular biology ·Vol. 10 ·No. 12 ·1990-12-00 ·Pages 6426-35

Aplan PD, Begley CG, Bertness V, Nussmeier M, Ezquerra A, Coligan J, Kirsch IR

Abstract

We describe the structural organization of the human SCL gene, a helix-loop-helix family member which we believe plays a fundamental role in hematopoietic differentiation. The SCL locus is composed of eight exons distributed over 16 kb. SCL shows a pattern of expression quite restricted to early hematopoietic tissues, although in malignant states expression of the gene may be somewhat extended into later developmental stages. A detailed analysis of the transcript(s) arising from the SCL locus revealed that (i) the 5' noncoding portion of the SCL transcript, which resides within a CpG island, has a complex pattern of alternative exon utilization as well as two distinct transcription initiation sites; (ii) the 5' portions of the SCL transcript contain features that suggest a possible regulatory role for these segments; (iii) the pattern of utilization of the 5' exons is cell lineage dependent; and (iv) all of the currently studied chromosomal aberrations that affect the SCL locus either structurally or functionally eliminate the normal 5' transcription initiation sites. These data suggest that the SCL gene, and specifically its 5' region, may be a target for regulatory interactions during early hematopoietic development.

Related Genes
SCL
MeSH Terms
Amino Acid Sequence Base Sequence Blotting, Northern Blotting, Southern Cell Line Cloning, Molecular DNA/genetics,isolation & purification Dinucleoside Phosphates Exons Humans Molecular Sequence Data Multigene Family Oligonucleotide Probes Polymerase Chain Reaction Protein Biosynthesis Restriction Mapping TATA Box Transcription, Genetic
Chemicals
Dinucleoside Phosphates Oligonucleotide Probes cytidylyl-3'-5'-guanosine DNA
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Aplan P D
Navy Medical Branch, National Cancer Institute, Bethesda, Maryland 20814.
Begley C G
Bertness V
Nussmeier M
Ezquerra A
Coligan J
Kirsch I R
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.12.6426-6435.1990
Published
1990-12-00
Pages
6426-35
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC362919
Subset
IM
Databases
GENBANK
M61103, M61104, M61105, M61108, M63572, M63576, M63584, M63589
External Links
PubMed source DOI: 10.1128/mcb.10.12.6426-6435.1990 PMC Full Text View journal details
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