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PMID: 2247060 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Induction of yeast histone genes by stimulation of stationary-phase cells.

Molecular and cellular biology ·Vol. 10 ·No. 12 ·1990-12-00 ·Pages 6356-61

Drebot MA, Veinot-Drebot LM, Singer RA, Johnston GC

Abstract

In the cell cycle of the budding yeast Saccharomyces cerevisiae, expression of the histone genes H2A and H2B of the TRT1 and TRT2 loci is regulated by the performance of "start," the step that also regulates the cell cycle. Here we show that histone production is also subject to an additional form of regulation that is unrelated to the mitotic cell cycle. Expression of histone genes, as assessed by Northern (RNA) analysis, was shown to increase promptly after the stimulation, brought about by fresh medium, that activates stationary-phase cells to reenter the mitotic cell cycle. The use of a yeast mutant that is conditionally blocked in the resumption of proliferation at a step that is not part of the mitotic cell cycle (M.A. Drebot, G.C. Johnston, and R.A. Singer, Proc. Natl. Acad. Sci. 84:7948, 1987) showed that this increased gene expression that occurs upon stimulation of stationary-phase cells took place in the absence of DNA synthesis and without the performance of start. This stimulation-specific gene expression was blocked by the mating pheromone alpha-factor, indicating that alpha-factor directly inhibits expression of these histone genes, independently of start.

MeSH Terms
Cell Cycle Cell Division DNA, Fungal/genetics,isolation & purification Gene Expression Regulation, Fungal Genes, Fungal Histones/genetics Kinetics Mitosis Plasmids RNA, Fungal/genetics,isolation & purification Saccharomyces cerevisiae/cytology,genetics,growth & development
Chemicals
DNA, Fungal Histones RNA, Fungal
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Drebot M A
Department of Microbiology, Dalhousie University, Halifax, Nova Scotia, Canada.
Veinot-Drebot L M
Singer R A
Johnston G C
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.12.6356-6361.1990
Published
1990-12-00
Pages
6356-61
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC362911
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.10.12.6356-6361.1990 PMC Full Text View journal details
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