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PMID: 2243813 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Improved synthesis of full-length RNA probe at reduced incubation temperatures.

Nucleic acids research ·Vol. 18 ·No. 21 ·1990-11-11 ·Pages 6463

Krieg PA

Abstract

暂无摘要

MeSH Terms

DNA-Directed RNA Polymerases/metabolism Electrophoresis, Polyacrylamide Gel Indicators and Reagents Molecular Weight RNA Probes/chemical synthesis T-Phages/enzymology Temperature Transcription, Genetic

Chemicals

Indicators and Reagents RNA Probes DNA-Directed RNA Polymerases

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Krieg P A

Department of Zoology, University of Texas, Austin 78712.

References (2)

2 references, click to expand

In vitro RNA synthesis with SP6 RNA polymerase.
Methods Enzymol. 1987;155:397-415 PMID: 2828872
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
Nucleic Acids Res. 1984 Sep 25;12(18):7035-56 PMID: 6091052

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/18.21.6463

Published

1990-11-11

Pages

6463

Language

English

Region

England

NLM ID

0411011

PMCID

PMC332583

Subset

Grants

NICHD NIH HHS · HD25179 · United States

External Links

PubMed source DOI: 10.1093/nar/18.21.6463 PMC Full Text View journal details

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