Use of protein A-coated colloidal gold particles for immunoelectronmicroscopic localization of ACTH on ultrathin sections.

Histochemistry ·Vol. 60 ·No. 3 ·1979-04-12 ·Pages 317-20

Batten TF, Hopkins CR

Abstract

ACTH was localized in dissociated porcine adenohypophysial cells using a novel indirect EM immunocytochemical technique. Incubation of ultrathin resin sections in anti-ACTH was followed by incubation with protein A-coated colloidal gold particles. Protein A binds specifically to the Fc part of the IgG molecule, and thus the ACTH-containing secretory granules became labelled with electron-dense gold particles. With this method, the dissociated porcine ACTH cells was identified as containing numerous round or ovoid 170--300 nm secretory granules.

MeSH Terms

Adrenocorticotropic Hormone/isolation & purification Animals Antigen-Antibody Reactions Cells, Cultured Colloids Cytoplasmic Granules/analysis Gold Microscopy, Electron/methods Pituitary Gland, Anterior/ultrastructure Staphylococcal Protein A Swine

Chemicals

Colloids Staphylococcal Protein A Gold Adrenocorticotropic Hormone

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Batten T F

Hopkins C R

References (10)

10 references, click to expand

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An immunocolloid method for the electron microscope.
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Adsorption of horseradish peroxidase, ovomucoid and anti-immunoglobulin to colloidal gold for the indirect detection of concanavalin A, wheat germ agglutinin and goat anti-human immunoglobulin G on cell surfaces at the electron microscopic level: a new method, theory and application.
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Article Info

Journal

Histochemistry

Abbr.

Histochemistry

ISSN

0301-5564

DOI

10.1007/BF00500659

Published

1979-04-12

Pages

317-20

Language

English

Region

Germany

NLM ID

0411300

Subset

External Links

PubMed source DOI: 10.1007/BF00500659

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Use of protein A-coated colloidal gold particles for immunoelectronmicroscopic localization of ACTH on ultrathin sections.

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